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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Mismatch Repair
Overview
Product name
Anti-Exonuclease 1 antibody [266]
See all Exonuclease 1 products (9) ...
Description
Mouse monoclonal [266] to Exonuclease 1
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Mouse).
Positive control
MCF-7 and MAD109.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
266
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Mismatch Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 89 kDa). Incubate for 2 hours at room temperature.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
Tissue specificity
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
Developmental stage
Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
Post-translational
modifications
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
Cellular localization
Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
Target information above from: UniProt accessionQ9UQ84
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Exo 1 antibody
- Exo I antibody
- Exo1 antibody
see all
Database links
- Entrez Gene: 9156 Human
- Entrez Gene: 26909 Mouse
- Omim: 606063 Human
- SwissProt: Q9UQ84 Human
- SwissProt: Q9QZ11 Mouse
- Unigene: 498248 Human
- Unigene: 283046 Mouse
References for Anti-Exonuclease 1 antibody [266] (ab3307)
This product has been referenced in:
- Chowdhury Det al. The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death. Mol Cell 23:133-42 (2006).Read more (PubMed: 16818237) »
See 1 publication for this product
Publishing research using ab3307? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"