Recombinant Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4442(2)] to Exportin 4/XPO4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Exportin 4/XPO4 antibody [EPR4442(2)]
See all Exportin 4/XPO4 primary antibodies -
Description
Rabbit monoclonal [EPR4442(2)] to Exportin 4/XPO4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Exportin 4/XPO4 aa 1050-1150 (C terminal).
Database link: Q0VG75 -
Positive control
- Jurkat, HeLa, SW480, C6, Raw264.7 and PC12 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4442(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 127 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 127 kDa. |
Target
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Function
Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
Sequence similarities
Belongs to the exportin family. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 64328 Human
- Entrez Gene: 57258 Mouse
- Entrez Gene: 290280 Rat
- Omim: 611449 Human
- SwissProt: Q9C0E2 Human
- SwissProt: Q9ESJ0 Mouse
- Unigene: 725169 Human
- Unigene: 202747 Mouse
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Alternative names
- Exp4 antibody
- Exportin-4 antibody
- exportin4 antibody
see all
Images
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All lanes : Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : C6 cell lysates
Lane 5 : Raw264.7 cell lysates
Lane 6 : PC12 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 127 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133237 has been referenced in 1 publication.
- Balukoff NC et al. A translational program that suppresses metabolism to shield the genome. Nat Commun 11:5755 (2020). PubMed: 33188200