Recombinant Anti-Exportin-5 antibody [EPR8453] (ab131281)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8453] to Exportin-5
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Exportin-5 antibody [EPR8453]
See all Exportin-5 primary antibodies -
Description
Rabbit monoclonal [EPR8453] to Exportin-5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Exportin-5 aa 1150-1250. The exact sequence is proprietary.
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Positive control
- HeLa, K562 and 293T cell lysates. A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.39 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8453 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
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Notes |
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ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Predicted molecular weight: 136 kDa. |
Target
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Function
Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi).
Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP. -
Tissue specificity
Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the exportin family. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 57510 Human
- Entrez Gene: 72322 Mouse
- Entrez Gene: 363194 Rat
- Omim: 607845 Human
- SwissProt: Q9HAV4 Human
- SwissProt: Q924C1 Mouse
- Unigene: 203206 Human
- Unigene: 275039 Mouse
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Alternative names
- Exp 5 antibody
- Exp5 antibody
- Exportin 5 antibody
see all
Images
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All lanes : Anti-Exportin-5 antibody [EPR8453] (ab131281) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 136 kDa -
Immunofluorescence analysis of A431 cells labelling Exportin-5 with ab131281 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab131281 has been referenced in 4 publications.
- Baldini E et al. Dysregulation of microRNA expression in diabetic skin. J Dermatol Sci 98:186-194 (2020). PubMed: 32402513
- Sherman Horev H et al. Increase in Processing Factors Is Involved in Skewed MicroRNA Expression in Patients with Ulcerative Colitis Who Develop Small Intestine Inflammation after Pouch Surgery. Inflamm Bowel Dis 24:1045-1054 (2018). PubMed: 29688474
- Ghatak S et al. Barrier Function of the Repaired Skin Is Disrupted Following Arrest of Dicer in Keratinocytes. Mol Ther 23:1201-1210 (2015). PubMed: 25896246
- Xie M et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). PubMed: 24360278