You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Ezrin 293T Transfected Lysate - (positive control) (ab94091)

Submit a Question

Overview

  • Product nameEzrin 293T Transfected Lysate - (positive control)
  • General notesab94091 is a 293T cell transfected lysate in which Human Ezrin has been transiently over-expressed using a pCMV-Ezrin. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Tissue specificity: Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. Similarity: Contains 1 FERM domain. Developmental stage: Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. PTM: Phosphorylated by tyrosine-protein kinases.

    • Applications

    Our Abpromise guarantee covers the use of ab94091 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Ezrin 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Ezrin 293T Transfected Lysate - (positive control) (ab94091)
      SDS-PAGE - Ezrin 293T Transfected Lysate - (positive control) (ab94091)
      ab94091 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Ezrin 293T Transfected Lysate - (positive control) (ab94091)
      Western blot - Ezrin 293T Transfected Lysate - (positive control) (ab94091)
      All lanes : Anti-Ezrin antibody (ab76872) at 1/500 dilution

      Lane 1 : Ezrin 293T Transfected Lysate - (positive control) (ab94091)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Ezrin 293T Transfected Lysate - (positive control) (ab94091)

    ab94091 has not yet been referenced specifically in any publications.

    Publishing research using ab94091 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab94091

    There are currently no Questions for ab94091.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"