Overview

  • Product nameAnti-F-actin antibody [NH3]
    See all F-actin primary antibodies
  • Description
    Mouse monoclonal [NH3] to F-actin
  • SpecificityNH3 recognises human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
  • Tested applicationsFlow Cyt, ICC/IF, IHC-R, WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Human monocytes and U397 cell line.

  • EpitopeThis antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
  • Positive control
    • gastrointestinal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.
ICC/IF Use at an assay dependent concentration. PubMed: 20798686
IHC-R Use at an assay dependent concentration.
WB 1/100 - 1/500. Detects a band of approximately 43 kDa. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
ELISA 1/10.
IHC-Fr Use at an assay dependent dilution.

Target

Anti-F-actin antibody [NH3] images

  • Anti-F-actin antibody [NH3] (ab205) at 1/100 dilution + Human pancreatic cancer cell line, whole cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-mouse antibody at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab205 staining F-actin in rat brain tissue by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 2% BSA for 1 hour followed by incubation with the primary antibody at a 1/25 dilution for 24 hours. A biotin-conjugated goat anti-mouse IgM was used as secondary antibody at a 1/400 dilution. No permeabilisation step was used.

References for Anti-F-actin antibody [NH3] (ab205)

This product has been referenced in:
  • Li QQ  et al. Involvement of NF-?B/miR-448 regulatory feedback loop in chemotherapy-induced epithelial-mesenchymal transition of breast cancer cells. Cell Death Differ 18:16-25 (2011). ICC/IF ; Human . Read more (PubMed: 20798686) »
  • Nohara A  et al. Cyclin-dependent Kinase-5 Is a Key Molecule in Tumor Necrosis Factor-{alpha}-induced Insulin Resistance. J Biol Chem 286:33457-65 (2011). WB . Read more (PubMed: 21813649) »

See all 8 Publications for this product

Product Wall

Je peux vous proposer ab205, Anti-F-actin antibody [NH3] qui est un monoclonal de souris garanti de fonctionner en immunofluorescence. Cependant, nous n’avons pas testé le taux de toxine dans ce produit et donc nous ne pouvons pas garantir...

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Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Tissue lysate - whole (CNS)
Specification CNS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Aug 01 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Resin sections)
Sample Mouse Tissue sections (Bone Tissue Cells)
Specification Bone Tissue Cells
Username

Abcam user community

Verified customer

Submitted Jul 10 2013

Thank you for your message and for providing this further information.
I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrang...

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Thank you for taking the time to provide additional information and to include our suggestions in your experiments. I appreciate the time that was spent doing additional experiments. I am sorry to hear that the results obtained are still not satisfacto...

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Thank you for contacting us.
I am sorry to hear you have been experiencing problems with one of our Anti-F-actin antibody [NH3] (ab205). The quality of our products is important to us and I would like to reassure you that we investigate all custom...

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Thank you for contacting us.

Unfortunately, we have not determined the pI (isoelectric) value for this product, ab205 Anti-F-actin antibody [NH3], nor any relevant goat anti-mouse IgG conjugated FITC antibodies like ab97022 and ab97039.
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Thank you for your enquiry regarding ab205 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I have few further questions to ask for better understan...

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Thank you very much for contacting Abcam about ab205.
I have heard back from the lab and they have told me that we did not test this antibody against G-actin as it only exists stably in a test tube. At physiological conditions, it begins to form F...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number **** for the e-cadherin antibody, ab53033.


Plea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"