Overview
- Product nameAnti-F-actin antibody [NH3]See all F-actin primary antibodies ...
- DescriptionMouse monoclonal [NH3] to F-actin
- SpecificityNH3 recognises human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
- Tested applicationsFlow Cyt, ICC/IF, WB, ELISA, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Human
- Immunogen
Human monocytes and U397 cell line.
- EpitopeThis antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
- Positive controlgastrointestinal tissue
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS with 0.1% sodium azide
-
Concentration information loading... - PurityPurified IgM
- Purification notesPurified IgM prepared from tissue culture supernatant.
- Clonality Monoclonal
- Clone numberNH3
- MyelomaNS1
- IsotypeIgM
- Light chain typeunknown
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt | Flow Cyt: 1/10. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 20798686 |
| WB | WB: 1/100 - 1/500. Detects a band of approximately 43 kDa. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. |
| ELISA | ELISA: 1/10. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
-
Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- ACTB antibodyACTB_HUMAN antibodyActin, cytoplasmic 1, N-terminally processed antibody
- Beta-actin antibodyf actin antibodyFilamentous actin antibody
see all
Anti-F-actin antibody [NH3] images
-
![Western blot - F-actin antibody [NH3] (ab205)](http://a.abcam.com/ps/datasheet/Images/0/ab205/ab205_2.jpg)
Western blot - F-actin antibody [NH3] (ab205)Anti-F-actin antibody [NH3] (ab205) at 1/100 dilution + Human pancreatic cancer cell line, whole cell lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse antibody at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteThis image is courtesy of an anonymous Abreview
-
![Immunohistochemistry (Frozen sections) - F-actin antibody [NH3] (ab205)](http://a.abcam.com/ps/datasheet/images/0/ab205/F-actin-Primary-antibodies-ab205-1.jpg)
Immunohistochemistry (Frozen sections) - F-actin antibody [NH3] (ab205)Image courtesy of an Abreview submitted by Jamie Richards.ab205 staining F-actin in rat brain tissue by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 2% BSA for 1 hour followed by incubation with the primary antibody at a 1/25 dilution for 24 hours. A biotin-conjugated goat anti-mouse IgM was used as secondary antibody at a 1/400 dilution. No permeabilisation step was used.
References for Anti-F-actin antibody [NH3] (ab205)
This product has been referenced in:
- Li QQ et al. Involvement of NF-?B/miR-448 regulatory feedback loop in chemotherapy-induced epithelial-mesenchymal transition of breast cancer cells. Cell Death Differ 18:16-25 (2011). ICC/IF ; Human . Read more (PubMed: 20798686) »
- Nohara A et al. Cyclin-dependent Kinase-5 Is a Key Molecule in Tumor Necrosis Factor-{alpha}-induced Insulin Resistance. J Biol Chem 286:33457-65 (2011). WB . Read more (PubMed: 21813649) »
