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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Anti-F4/80 antibody [BM8] (FITC) (ab60343)
Product partner
Related products
- Secondary antibodies
- Isotype controls
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab60343? Please let us know so that we can cite the reference in this datasheet
ab60343 has been referenced in 3 publications.
- Koenen TB et al. The inflammasome and caspase-1 activation: a new mechanism underlying increased inflammatory activity in human visceral adipose tissue. Endocrinology 152:3769-78 (2011). PubMed: 21862623
- Church JE et al. Early functional muscle regeneration after myotoxic injury in mice is unaffected by nNOS absence. Am J Physiol Regul Integr Comp Physiol 301:R1358-66 (2011). PubMed: 21849632
- Duann P & Lianos EA GEC-targeted HO-1 expression reduces proteinuria in glomerular immune injury. Am J Physiol Renal Physiol 297:F629-38 (2009). IHC-Fr; Mouse. PubMed: 19587144
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - F4/80 antibody [BM8] (FITC) (ab60343)](/ps/datasheet/images/60/ab60343/F480-Primary-antibodies-ab60343-1.jpg)
Immunocytochemistry/ Immunofluorescence - F4/80 antibody [BM8] (FITC) (ab60343)
ICC/IF image of ab60343 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1µg/ml, FITC conjugated) overnight at +4ºC. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry - F4/80 antibody [BM8] (FITC) (ab60343)](/ps/datasheet/images/60/ab60343/F480-Primary-antibodies-ab60343-2.jpg)
Flow Cytometry - F4/80 antibody [BM8] (FITC) (ab60343)
Overlay histogram showing RAW 264.7 cells stained with ab60343 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1/100 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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