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ab16911 has been referenced in 14 publications.
Publishing research using ab16911? Please let us know so that we can cite the reference in this datasheet
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ab16911 staining mouse spleen tissue sections by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed without permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 16 hour at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/500 was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Miss Silke Vorwald
ab16911 staining F4/80 in Mouse brain cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 1 hour at 20ºC. Samples were incubated with primary antibody (1/250) for 16 hours at 4ºC. An Alexa Fluor®568-conjugated Goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Overlay histogram showing HeLa cells stained with ab16911 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab16911 staining F4/80 on macrophages in mouse liver tissue by Immunohistochemistry (Frozen sections).
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