F4/80 antibody [CI:A3-1] (ab6640)

Product Name 

F4/80 antibody [CI:A3-1]

Product type 

Primary antibodies

Description 

Rat monoclonal [CI:A3-1] to F4/80

Immunogen 

Thioglycollate stimulated peritoneal macrophages from C57/BL mice

Reacts with 
(species key)

Ms

Specificity 

This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC (PFA fixed), IHC-FoFr, IHC-Fr, IHC-P, IHC-R, IP, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use 10µl for 10⁵ cells.
IHC-P: Use at a concentration of 10 µg/ml. This antigen is known to be sensitive to fixation: Trypsin or Proteinase K (6 min, RT) enzyme pretreatment is recommended for tissues fixed for less than 24 hours. Citrate buffer pH6.0 is recommended for tissues fixed for more than 24 hours.
IHC-Fr: Use at a concentration of 10 µg/ml.
IHC (PFA fixed): Use at a concentration of 10 µg/ml.
IHC-FoFr: Use at a concentration of 10 µg/ml.
IHC-R: Use at a concentration of 10 µg/ml.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at a concentration of 10 µg/ml. Use under non reducing conditions. Detects a band of approximately 160 kDa (predicted molecular weight of precursor protein: 102 kDa; protein is heavily glycosylated). Block in 5% milk for 1 hour.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse spleen and macrophages

Cellular localization 

Membrane; multi pass membrane protein.

Research areas 

Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Immunology >> Innate Immunity >> Macrophage / Inflamm.

Relevance 

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins which shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

CI:A3-1

Myeloma 

NS1

Isotype 

IgG2b

Purity 

IgG fraction

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for F4/80 antibody [CI:A3-1] images, references, products related to ab6640 and other tools.

F4/80 antibody [CI:A3-1] images:

References for F4/80 antibody [CI:A3-1] (ab6640)

  This product has been used in: (two most recent references)

Lee TY et al. Angiostatin regulates the expression of antiangiogenic and proapoptotic pathways via targeted inhibition of mitochondrial proteins. Blood 114:1987-98 (2009). IHC-Fr; Mouse. PubMed: 19465692

Lu X & Kang Y Chemokine (C-C motif) ligand 2 engages CCR2+ stromal cells of monocytic origin to promote breast cancer metastasis to lung and bone. J Biol Chem 284:29087-96 (2009). IHC-P; Mouse. PubMed: 19720836

See all 46 publication references for this product.


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