Overview
- Product nameAnti-F4/80 antibody [CI:A3-1]See all F4/80 primary antibodies ...
- DescriptionRat monoclonal [CI:A3-1] to F4/80
- SpecificityThis antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
- Tested applicationsWB, IP, IHC-P, RIA, IHC-Fr, Flow Cyt, ICC, IHC-FoFr, ICC/IF, IHC-R more details
- Species reactivityReacts with: Mouse
- Immunogen
Thioglycollate stimulated peritoneal macrophages from C57/BL mice
- Positive controlMouse spleen and macrophages
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
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Concentration information loading... - PurityIgG fraction
- Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Clonality Monoclonal
- Clone numberCI:A3-1
- MyelomaNS1
- IsotypeIgG2b
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 10 µg/ml. Use under non reducing condition. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa). (predicted molecular weight of precursor protein: 102 kDa; protein is heavily glycosylated). Block in 5% milk for 1 hour. |
| IP | IP: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval (e.g. with citrate buffer) or carry out Proteinase K pre-treatment (starting treatment time 3min). |
| RIA | RIA: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use a concentration of 10 µg/ml. |
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
| ICC | ICC: Use at an assay dependent concentration. |
| IHC-FoFr | IHC-FoFr: Use a concentration of 10 µg/ml. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| IHC-R | IHC-R: Use a concentration of 10 µg/ml. |
Target
- FunctionCould be involved in cell-cell interactions.
- Tissue specificityWide expression; increased levels in peripheral blood mononuclear cells.
- Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. - Cellular localizationCell membrane.
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
Target information above from: UniProt accession
Q14246
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cell surface glycoprotein EMR1 antibodyCell surface glycoprotein F4/80 antibodyDD7A5 7 antibody
- Egf like module containing mucin like hormone receptor like 1 antibodyEgf like module containing mucin like hormone receptor like sequence 1 antibodyEGF like module receptor 1 antibodyEGF TM7 antibodyEGF-like module receptor 1 antibodyEGF-like module-containing mucin-like hormone receptor-like 1 antibodyEGFTM7 antibodyEMR 1 antibodyEMR1 antibodyEMR1 hormone receptor antibodyEMR1_HUMAN antibodyGpf480 antibodyLy71 antibodyLymphocyte antigen 71 antibodyTM7LN3 antibody
see all
Anti-F4/80 antibody [CI:A3-1] images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - F4/80 antibody [CI:A3-1] (ab6640)](http://a.abcam.com/ps/datasheet/images/6/ab6640/F480-Primary-antibodies-ab6640-26.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - F4/80 antibody [CI:A3-1] (ab6640)This image is courtesy of an Anonymous Abreview.ab6640 staining F4/80 in Mouse skeletal muscle tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to Heat-mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1% BSA for 25 minutes at 25°C. The primary antibody was diluted 1/100 in 1%BSA/PBS and incubated with the sample for 1 hour 30 minutes at 25°C. An HRP polymer-conjugated Goat anti-Rat polyclonal was used as the secondary antibody. -
![Immunohistochemistry (PFA perfusion fixed frozen sections) - F4/80 antibody [CI:A3-1] (ab6640)](http://a.abcam.com/ps/datasheet/Images/6/ab6640/ab6640_5.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - F4/80 antibody [CI:A3-1] (ab6640)This image is courtesy of an anonymous Abreviewab6640 staining mouse brain tissue sections by IHC-FoFr. Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/10 and incubated with the sample for 18 hours at 4°C. A biotinylated goat anti-rat antibody, diluted 1/500, was used as the secondary. This image demonstrates quiescent microglia in the normal mouse brain (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - F4/80 antibody [CI:A3-1] (ab6640)](http://a.abcam.com/ps/datasheet/images/6/ab6640/F480-Primary-antibodies-ab6640-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - F4/80 antibody [CI:A3-1] (ab6640)This image is a courtesy of Sarka Lhotakab6640 staining F4/80 in mouse adipose tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation and blocking with 5% serum was performed for 20 minutes at 20°C. The primary antibody was diluted 1/150 and incubated with sample in Tris + 5 % normal rabbit serum for 1 hour at 20°C. A Biotin conjugated rabbit polyclonal to rat IgG was used at dilution at 1/50 as secondary antibody. The antibody stained interstitial macrophages in the adipose tissue of lean mice (A), and macrophage associated with "crown-like structure" in mice fed high-fat diet (B).
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![Immunocytochemistry/ Immunofluorescence - F4/80 antibody [CI:A3-1] (ab6640)](http://a.abcam.com/ps/datasheet/images/6/ab6640/F480-Primary-antibodies-ab6640-6.jpg)
Immunocytochemistry/ Immunofluorescence - F4/80 antibody [CI:A3-1] (ab6640)This image is courtesy of an anonymous Abreviewab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody. -
![Western blot - F4/80 antibody [CI:A3-1] (ab6640)](http://a.abcam.com/ps/datasheet/images/6/ab6640/F480-Primary-antibodies-ab6640-8.jpg)
Western blot - F4/80 antibody [CI:A3-1] (ab6640)Anti-F4/80 antibody [CI:A3-1] (ab6640) at 1/1000 dilution (for 8 hours at 4°C) + Mouse pancreatic cancer whole tissue lysate. at 25 µg
Secondary
An HRP-conjugated Goat anti-rat IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 102 kDa
Observed band size : ~130 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 minutesThis image is courtesy of an Abreview submitted by Pawel Mazur
Protocols
References for Anti-F4/80 antibody [CI:A3-1] (ab6640)
This product has been referenced in:
- Song P et al. Emodin regulates glucose utilization by activating AMP-activated protein kinase. J Biol Chem 288:5732-42 (2013). IHC-P ; Mouse . Read more (PubMed: 23303186) »
- Yang X et al. Mechanism of fibrotic cardiomyopathy in mice expressing truncated Rho-associated coiled-coil protein kinase 1. FASEB J 26:2105-16 (2012). ICC/IF ; Mouse . Read more (PubMed: 22278938) »
