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ab74382
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab74383? Please let us know so that we can cite the reference in this datasheet
ab74383 has been referenced in 1 publications.
- DeClercq V et al. Dietary t10,c12-CLA but not c9,t11 CLA reduces adipocyte size in the absence of changes in the adipose renin-angiotensin system in fa/fa Zucker rats. Lipids 45:1025-33 (2010). WB; Rat. PubMed: 20844975
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - F4/80 antibody (ab74383)
All lanes : Anti-F4/80 antibody (ab74383) at 1 µg/ml
Lane 1 : Spleen (Mouse) Tissue Lysate
Lane 2 : Thymus (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate (ab7935)
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 102 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 16 minutes
F4/80 has a predicted molecular weight of 102 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data).
F4/80 antibody for Western blot in Mouse (74383)
F4/80 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (74383)
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