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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-F4/80 antibody
See all F4/80 products (14) ...
Description
Rabbit polyclonal to F4/80
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Dog
Immunogen
Synthetic peptide derived from within residues 900 to the C-terminus of Mouse F4/80.
(Peptide available as ab74382.)
Positive control
This antibody gave a positive signal in RAW 264.7 cells and in the following Mouse tissue lysates: Spleen; Thymus; Lung.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Western blot - F4/80 antibody (ab74383)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab74383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 102 kDa).
Target
Function
Could be involved in cell-cell interactions.
Tissue specificity
Wide expression; increased levels in peripheral blood mononuclear cells.
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ14246
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell surface glycoprotein EMR1 antibody
- Cell surface glycoprotein F4/80 antibody
- DD7A5 7 antibody
see all
Database links
- Entrez Gene: 485015 Dog
- Entrez Gene: 13733 Mouse
- Entrez Gene: 316137 Rat
- SwissProt: Q61549 Mouse
- SwissProt: Q5Y4N8 Rat
- Unigene: 2254 Mouse
- Unigene: 205884 Rat
Anti-F4/80 antibody images:
Western blot - F4/80 antibody (ab74383)
All lanes : Anti-F4/80 antibody (ab74383) at 1 µg/ml
Lane 1 : Spleen (Mouse) Tissue Lysate
Lane 2 : Thymus (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate (ab7935)
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 102 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 16 minutes
F4/80 has a predicted molecular weight of 102 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data).
References for Anti-F4/80 antibody (ab74383)
This product has been referenced in:
- DeClercq Vet al. Dietary t10,c12-CLA but not c9,t11 CLA reduces adipocyte size in the absence of changes in the adipose renin-angiotensin system in fa/fa Zucker rats. Lipids 45:1025-33 (2010). WB; Rat.Read more (PubMed: 20844975) »
See 1 publication for this product
Publishing research using ab74383? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"