Recombinant Anti-F4/80 antibody [SP115] (ab111101)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP115] to F4/80
- Suitable for: IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-F4/80 antibody [SP115]
See all F4/80 primary antibodies -
Description
Rabbit monoclonal [SP115] to F4/80 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse colon, liver and lung tissue; M1 and M2 macrophages from mice colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (11) |
1/50 - 1/100.
Incubate primary antibody overnight at 4C. For antigen retrieval: Boil tissue section in Tris-EDTA (pH 9.0) buffer for 10 min followed by cooling at RT for 20 min. Abcam recommends using a polymer-HRP conjugated secondary for optimal signal. |
Notes |
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IHC-P
1/50 - 1/100. Incubate primary antibody overnight at 4C. For antigen retrieval: Boil tissue section in Tris-EDTA (pH 9.0) buffer for 10 min followed by cooling at RT for 20 min. Abcam recommends using a polymer-HRP conjugated secondary for optimal signal. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling F4/80 with ab111101 at 1/250 dilution (0.48 µg/ml). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on macrophages in the mouse liver.
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Representative immunostaining of F4/80-positive macrophages in the distal colon from healthy and colitic mice treated with and without enoxaparin.
For immunohistochemical staining, antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8 or 10 mM citrate buffer, pH 6. Activity of endogenous peroxidase was blocked by incubating sections with 3% v/v hydrogen for 20 minutes. Sections were then washed with 0.05 M Tris-buffered saline containing 0.5% v/v Tween 20 (TBST), pH 7.6. Subsequently, sections were incubated with serum-free protein block for 10 minutes. Colon sections were then incubated with primary antibody ab111101 at 1/100 dilution overnight at 4°C or room temperature for 1 hour. Sections were then washed 3 x 5 minutes and allowed to react with secondary antibody: anti-rabbit immunoglobulin C conjugated to horseradish peroxidase (HRP) (ab7090) at 1/300 dilution at room temperature for 1 hour.
Scale bar = 100 μm for 400 x magnification. Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.
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Representative images of (A) M1 macrophages (F4/80+ and iNOS+) and (B) M2 macrophages (F4/80+ and CD206+) using colon tissue from n = 3–5 mice. F4/80 positive cells were visualized using Alexa Fluor 594-conjugated goat anti-rat IgG (red). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue).
Scale bar = 50 μm for 400 × magnification. Control, C; untreated colitis, DSS; colitis with oral enoxaparin, DSS+OE.
For immunofluorescence staining, sections were dewaxed and rehydrated before antigen retrieval using 10 mM citrate buffer, pH 6 for 15 minutes at 97°C. Sections were incubated with serum-free protein block and permeabilized with 0.4% v/v Triton-X at room temperature for 30 minutes. Sections were incubated with primary antibodies anti-F4/80 (ab16911) at 1/25 dilution overnight at 4°C or at room temperature for 1 hour. Sections were washed with TBST 3 × 10 minutes and incubated with species-specific secondary antibodies: anti-rat IgG H&L AlexaFluor 594 (ab150160, Abcam, 1:1000) and anti-rabbit IgG H&L AlexaFluor 488 (A11070, Thermo Fisher Scientific, Melbourne, Australia, 1:1000) at room temperature for 2 hours. Sections were rinsed with TBST 3 × 10 minutes, followed by a quick wash with distilled water before mounting using Glycerol Mounting Medium (Abcam) that contained 4’,6-diamidino-2-phenylindole (DAPI) and 1,4-diazobicyclo-2,2,2-octane (DABCO). Labelled tissues were visualized using a Leica DM LB2 microscope. Fluorescence images (400 × magnification) were captured using NIS-Elements 4.13 (Nikon) software.
For full image see PMID: 26218284.
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ab111101 at 1/100 dilution staining F4/80 in Formalin-fixed, paraffin-embedded Mouse liver tissue.
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Immunohistochemistry analysis of Formalin fixed paraffin-embedded mouse lung tissue sections labeling F4/80 with ab111101 at 1/200 for 16 hours at 4°C. Biotin conjugated Goat anti-rabbit polyclonal antibody at 1/500 was used as the secondary. Antigen retrieval was heat mediated using citrate buffer pH 6.0.
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Immunohistochemical analysis staining for macrophages in (A) mouse uterus and (B) mouse spleen using ab111101 at a dilution of 1:200. HRP Anti-Rabbit IgG (Peroxidase) Polymer D antibody was used as a secondary.
Datasheets and documents
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SDS download
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Datasheet download
References (120)
ab111101 has been referenced in 120 publications.
- Chen GT et al. Disruption of β-Catenin-Dependent Wnt Signaling in Colon Cancer Cells Remodels the Microenvironment to Promote Tumor Invasion. Mol Cancer Res 20:468-484 (2022). PubMed: 34799404
- Kleefeldt F et al. Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesis. Cell Death Dis 13:220 (2022). PubMed: 35264563
- Ni X et al. Carabin Deficiency Aggravates Hepatic Ischemia-Reperfusion Injury Through Promoting Neutrophil Trafficking via Ras and Calcineurin Signaling. Front Immunol 13:773291 (2022). PubMed: 35265067
- Jeon SH et al. Beneficial Activities of Alisma orientale Extract in a Western Diet-Induced Murine Non-Alcoholic Steatohepatitis and Related Fibrosis Model via Regulation of the Hepatic Adiponectin and Farnesoid X Receptor Pathways. Nutrients 14:N/A (2022). PubMed: 35277054
- Yang Y et al. HPS protects the liver against steatosis, cell death, inflammation, and fibrosis in mice with steatohepatitis. FEBS J 289:5279-5304 (2022). PubMed: 35285180