Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Overview
Product name
Anti-FA2H antibody
See all FA2H products (2) ...
Description
Mouse polyclonal to FA2H
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length human FA2H protein (AAH17049.2)
Positive control
FA2H transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Lipid metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
-
Western blot - FA2H antibody (ab68902)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).
Target
Function
Required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids.
Tissue specificity
Detected in differentiating cultured keratinocytes (at protein level). Detected in epidermis and cultured keratinocytes. Highly expressed in brain and colon. Detected at lower levels in testis, prostate, pancreas and kidney.
Involvement in disease
Defects in FA2H are the cause of leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia (DLDSP) [MIM:612443]. The disorder consists of a progressive neurologic disease manifested by spasticity, disordered tonicity of muscle, and white matter degeneration.
Defects in FA2H are a cause of spastic paraplegia autosomal recessive type 35 (SPG35) [MIM:612319]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Sequence similarities
Belongs to the sterol desaturase family. SCS7 subfamily.
Contains 1 cytochrome b5 heme-binding domain.
Domain
The histidine box domains may contain the active site and/or be involved in metal ion binding.
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane.
Target information above from: UniProt accessionQ7L5A8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FA2H antibody
- FA2H_HUMAN antibody
- FAAH antibody
see all
Database links
- Entrez Gene: 79152 Human
- Omim: 611026 Human
- SwissProt: Q7L5A8 Human
- Unigene: 461329 Human
Anti-FA2H antibody images:
Western blot - FA2H antibody (ab68902)
All lanes : Anti-FA2H antibody (ab68902) at 1/500 dilution
Lane 1 : FA2H transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
References for Anti-FA2H antibody (ab68902)
ab68902 has not yet been referenced specifically in any publications.
Publishing research using ab68902? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"