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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab54615? Please let us know so that we can cite the reference in this datasheet
ab54615 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunoprecipitation - Anti-FAAH1 antibody (ab54615)
FAAH1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to FAAH1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54615.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 63kDa: FAAH1.
Western blot - Anti-FAAH1 antibody (ab54615)
Predicted band size : 63 kDa
FAAH antibody (ab54615) at 1ug/lane + A-431 cell lysate at 25ug/lane.
Western blot - Anti-FAAH1 antibody (ab54615)
Anti-FAAH1 antibody (ab54615) + Rat Brain
Predicted band size : 63 kDa
Western blot - Anti-FAAH1 antibody (ab54615)
Anti-FAAH1 antibody (ab54615) + PC-12
Predicted band size : 63 kDa
Western blot - Anti-FAAH1 antibody (ab54615)
Anti-FAAH1 antibody (ab54615) + NIH/3T3
Predicted band size : 63 kDa
FAAH antibody for WB in Mouse (54615)
FAAH antibody for IHC-P in Rat (54615)
FAAH antibody for IHC-P in Human (54615)
FAAH antibody for IHC-P in Mouse (54615)
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