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Anti-FAAP24 antibody (ab48124)

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    Overview

    Product name

    Anti-FAAP24 antibody
    See all FAAP24 products (2) ...

    Description

    Rabbit polyclonal to FAAP24

    Tested applications

    WBmore details

    Cross reactivity

    Predicted to work with

    Mouse, Human

    Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FAAP24.

    (Peptide available as ab49605.)

    Positive control

    This antibody gave a positive signal in HeLa Nuclear extract, Jurkat Nuclear extract and Irradiated HeLa whole cell lysate.

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4

    Concentration

    Concentration information loading...

    Purity

    Immunogen affinity purified

    Clonality

    Polyclonal

    Isotype

    IgG

    • Western blot - Anti-FAAP24 antibody (ab48124)Western blot - Anti-FAAP24 antibody (ab48124) image (enlarge)

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab48124 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Target

    Relevance

    FAAP24 was identified as a protein that interacts with FANCM as part of the core Fanconi Anemia complex. It is homologous to the XPF family of endonucleases. FAAP24 is required for normal levels of FANCD2 ubiquitination after DNA damage. Knockdown of FAAP24 leads to chromosomal instability.

    Cellular localization

    Nuclear

    Alternative names

    • C19ORF40 antibody
    • MGC32020 antibody

    Database links

    Anti-FAAP24 antibody images:

      Western blot - Anti-FAAP24 antibody (ab48124)

    Western blot - Anti-FAAP24 antibody (ab48124)

    All lanes : Anti-FAAP24 antibody (ab48124) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HeLa Whole Cell Lysate - Irradiated (5Gy

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    References for Anti-FAAP24 antibody (ab48124)

    ab48124 has not yet been referenced specifically in any publications.

    Publishing research using ab48124? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"