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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
Overview
Product name
Anti-FAAP24 antibody
See all FAAP24 products (2) ...
Description
Rabbit polyclonal to FAAP24
Tested applications
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FAAP24.
(Peptide available as ab49605.)
Positive control
This antibody gave a positive signal in HeLa Nuclear extract, Jurkat Nuclear extract and Irradiated HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Western blot - Anti-FAAP24 antibody (ab48124)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab48124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
Target
Relevance
FAAP24 was identified as a protein that interacts with FANCM as part of the core Fanconi Anemia complex. It is homologous to the XPF family of endonucleases. FAAP24 is required for normal levels of FANCD2 ubiquitination after DNA damage. Knockdown of FAAP24 leads to chromosomal instability.
Cellular localization
Nuclear
Alternative names
- C19ORF40 antibody
- MGC32020 antibody
Database links
- Entrez Gene: 91442 Human
- SwissProt: Q9BTP7 Human
Anti-FAAP24 antibody images:
Western blot - Anti-FAAP24 antibody (ab48124)
All lanes : Anti-FAAP24 antibody (ab48124) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HeLa Whole Cell Lysate - Irradiated (5Gy
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 24 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 120 kDa,40 kDa. We are unsure as to the identity of these extra bands.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
References for Anti-FAAP24 antibody (ab48124)
ab48124 has not yet been referenced specifically in any publications.
Publishing research using ab48124? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"