Anti-FABP4 antibody (ab13979)

Phone call reporting non-specific staining using anti-FABP4 antibody (ab13979).

ANSWER:

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I amattaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. OnceI have receive the completed questionnaire,I will look at the protocol and see if there are any suggestionsI can make that may improve the results. This information will also allow us to investigate this case internally and initiate any additional testing where necessary.

If the product was purchased in the last six months andwas being used according to our Abpromise, we would be happy to replace or refund the antibody.

As discussed over the phone, I am looking into exactly what protocol has been used with this antibody previously and if we have any other suitable alternatives for you.

I look forward to receiving your reply.

Hi, As to FABP4 antibody (ab13979), I'd like to know that whetehr this antibody works in zebrafish. May I know what the sequence of the peptide you used to immunize the rabbit?

ANSWER:

Thank you for your enquiry.

The immunogen used to make this antibody was not a peptide but the full length protein.

SwissProt link (http://www.uniprot.org/uniprot/P15090)

I have taken the liberty BLASTing the sequence with zebrafish to check for homology (see attached PDF file). Unfortunately, there does not seem to be any hits for FABP4 protein and I can't be sure if this product could or could not work for zebrafish.

Please bear in mind that this product has not been tested to work in zebrafish, therefore we are unable to guarantee that you will be able to obtain good results. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts.

To find out more about our Abreview system, please see the following URL:

http://www.abcam.com/abreviews

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.