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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Overview
Product name
Anti-FACL4 antibody - Aminoterminal end
See all FACL4 products (3) ...
Description
Rabbit polyclonal to FACL4 - Aminoterminal end
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide selected from the N-terminal region of human FACL4.
Positive control
Human hepatocarcinoma tissue and 293 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects bands of approximately 28, 30 and 65 kDa (predicted molecular weight: 79 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
Involvement in disease
Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionO60488
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACS 4 antibody
- ACS4 antibody
- ACSL 4 antibody
see all
Database links
- Entrez Gene: 2182 Human
- Omim: 300157 Human
- SwissProt: O60488 Human
- Unigene: 268785 Human
Anti-FACL4 antibody - Aminoterminal end images:
Western blot - FACL4 antibody - Aminoterminal end (ab70937)
Anti-FACL4 antibody - Aminoterminal end (ab70937) at 1/100 dilution + 293 cell lysate at 12.5 µg
Predicted band size : 79 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa,30 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FACL4 antibody - Aminoterminal end (ab70937)
Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab70937 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
References for Anti-FACL4 antibody - Aminoterminal end (ab70937)
ab70937 has not yet been referenced specifically in any publications.
Publishing research using ab70937? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"