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Western blot - FACL4 antibody (ab38420)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FACL4 antibody (ab38420)
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Product Name
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FACL4 antibody
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See all FACL4 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FACL4
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Immunogen
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KLH conjugated synthetic peptide (10-30 aa in length) in the region of 276~292 of human FACL4.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Predicted molecular weight: 79 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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RKO cell lysate, hepatocarcinoma tissue.
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Cellular localization
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Cell Membrane
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Research areas
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Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Relevance
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Long chain acyl-CoA synthetase (LACS), or long chain fatty acid-CoA ligase (FACL), converts free long chain fatty acids into fatty acyl-CoA esters, key intermediates in the synthesis of complex lipids. The FACL4 gene encodes a form of LACS, is expressed in several tissues and is highly expressed in adult human brain, especially in the cerebellum and hippocampus. Research indicates that FACL4 is specifically expressed in neurons and not in glial cells. It has been suggested that the absence of FACL4 might play a role in the development of mental retardation or other signs associated with Alport syndrome.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FACL4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FACL4 antibody images, references, products related to ab38420 and other tools.
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FACL4 antibody images:
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 Western blot - FACL4 antibody (ab38420)
FACL4 antibody (ab38420) + RKO cell lysate (human colon cancer) treated with 10% FCS for 24hrs
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Drs. M.G. Sciulli and P. Patrignani, University of Chieti
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FACL4 antibody (ab38420)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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References for FACL4 antibody (ab38420)
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This product has been used in:
Simmen T et al. PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. EMBO J 24:717-29 (2005). PubMed: 15692567
If you publish research using ab38420 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FACL4
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FACL4 antibody - more information
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Customer reviews (feedback) regarding FACL4 antibody |
Customer FAQs regarding FACL4 antibody |
Protocols for FACL4 antibody |
Price and availability of products related to FACL4 antibody |
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FACL4 antibody - related products:
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FACL4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FACL4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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