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Read our guarantee »Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Anti-FACL4 antibody
See all FACL4 products (3) ...
Rabbit polyclonal to FACL4
ELISA, WB, IHC-Pmore details
Reacts with
Human
KLH conjugated synthetic peptide (10-30 aa in length) in the region of 276~292 of human FACL4.
RKO cell lysate, hepatocarcinoma tissue.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
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Protein G purified
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Polyclonal
IgG
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Our Abpromise guarantee covers the use of ab38420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 79 kDa.
IHC-P: 1/50 - 1/100.
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
Belongs to the ATP-dependent AMP-binding enzyme family.
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionO60488
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - FACL4 antibody (ab38420)

Anti-FACL4 antibody (ab38420) + RKO cell lysate (human colon cancer) treated with 10% FCS for 24hrs
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Drs. M.G. Sciulli and P. Patrignani, University of Chieti
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FACL4 antibody (ab38420)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ab38420 has not yet been referenced specifically in any publications.
Publishing research using ab38420? Please let us know so that we can cite the reference in this datasheet
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Anti-FACL4 antibody (ab38420) + RKO cell lysate (human colon cancer) treated with 10% FCS for 24hrs
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Drs. M.G. Sciulli and P. Patrignani, University of Chieti

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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