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Immunohistochemistry (Paraffin-embedded sections) - FADD antibody [64A6] (ab49444)
![Immunohistochemistry (Paraffin-embedded sections) - FADD antibody [64A6] (ab49444)](/ps/datasheet/images/NCL-FADD.jpg)
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Immunocytochemistry/ Immunofluorescence - FADD antibody [64A6] (ab49444)
![Immunocytochemistry/ Immunofluorescence - FADD antibody [64A6] (ab49444)](\ps\datasheet\images\49\ab49444\FADD-Primary-antibodies-ab49444-1.jpg)
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Product Name
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FADD antibody [64A6]
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See all FADD antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [64A6] to FADD
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Immunogen
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Prokaryotic recombinant protein corresponding to a 113 amino acid portion of the C terminal region of the FADD (Fas-associated death domain) protein (Human)
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Reacts with
(species key)
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Hu
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Specificity
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Human Fas-associated death domain protein.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/1000.
IHC-Fr: 1/25 - 1/50. Primary antibody incubation at 25°C for 60 minutes is recommended. (Acetone fixation recommended).
IHC-P: 1/100 - 1/200. Primary antibody incubation at 25°C for 60 minutes and high temperature antigen unmasking technique are recommended for enhanced results.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Testis and Human foetus.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> FADD
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Relevance
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FADD (Fas Associated Death Domain) is an apoptosis adapter molecule enabling transduction of the apoptosis signal initiated via the FasL/Fas receptor interaction. The protein contains a C terminal death domain that interacts with the Fas receptor death domain. The N terminus contains a death effectors domain (DED) which recruits caspase to the death inducing signaling complex (DISC) and initiates the apoptotic caspase cascade. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives its autoactivation through self-cleavage. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. FADD is implicated in non-apoptotic cellular pathways such as the regulation of cell cycle machinery in T lymphocytes. This is connected to the phosphorylation state of FADD and to the FasL/TRAIL induced transcriptional activation of cfos protooncogene. FADD also interacts with the hepatitis C virus core protein in the HEK 293 cell line.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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64A6
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Myeloma
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P3-NS1/1-Ag4-1
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FADD antibody [64A6] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FADD antibody [64A6] images, references, products related to ab49444 and other tools.
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FADD antibody [64A6] images:
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 Immunohistochemistry (Paraffin-embedded sections) - FADD antibody [64A6] (ab49444)
Immunohistochemical staining (Paraffin section) for FADD (Fas-associated death domain) protein using ab49444 with Human foetus as positive control. Note intense membrane and cytoplasmic staining of developing foetal skin, internal components and organs.
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Immunocytochemistry/ Immunofluorescence - FADD antibody [64A6] (ab49444)
ICC/IF image of ab49444 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49444, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to FADD
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FADD antibody [64A6] - more information
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Customer reviews (feedback) regarding FADD antibody [64A6] |
Customer FAQs regarding FADD antibody [64A6] |
Protocols for FADD antibody [64A6] |
Price and availability of products related to FADD antibody [64A6] |
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FADD antibody [64A6] - related products:
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FADD antibody [64A6] - other tools:
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Contact Abcam with a Technical Enquiry about FADD antibody [64A6] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Paraffin-embedded sections) - FADD antibody [64A6] (ab49444)](/index.html?datasheet=49444#NCL-FADD.jpg)
![Immunocytochemistry/ Immunofluorescence - FADD antibody [64A6] (ab49444)](/index.html?datasheet=49444#FADD-Primary-antibodies-ab49444-1.jpg)
