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Product Name
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FADD antibody [FD19]
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See all FADD antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [FD19] to FADD
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Dot, ELISA, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at a concentration of 0.5-1 µg/ml, determined using total cell extracts of A431 cells.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> FADD
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Relevance
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FADD (Fas Associated Death Domain) is an apoptosis adapter molecule enabling transduction of the apoptosis signal initiated via the FasL/Fas receptor interaction. The protein contains a C terminal death domain that interacts with the Fas receptor death domain. The N terminus contains a death effectors domain (DED) which recruits caspase to the death inducing signaling complex (DISC) and initiates the apoptotic caspase cascade. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives its autoactivation through self-cleavage. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. FADD is implicated in non-apoptotic cellular pathways such as the regulation of cell cycle machinery in T lymphocytes. This is connected to the phosphorylation state of FADD and to the FasL/TRAIL induced transcriptional activation of cfos protooncogene. FADD also interacts with the hepatitis C virus core protein in the HEK 293 cell line.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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FD19
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Myeloma
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NS1
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Isotype
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IgG1
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FADD antibody [FD19] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to FADD
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FADD antibody [FD19] - more information
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