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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-FADD antibody [FD19]
See all FADD products (10) ...
Description
Mouse monoclonal [FD19] to FADD
Tested applications
ELISA, Dot Blot, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
FD19
Myeloma
NS1
Isotype
IgG1
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> FADD
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Dot Blot
IP
Application notes
Dot: Use at a concentration of 0.5-1 µg/ml, determined using total cell extracts of A431 cells.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificity
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similarities
Contains 1 death domain.
Contains 1 DED (death effector) domain.
Domain
Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
Target information above from: UniProt accessionQ13158
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Database links
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
References for Anti-FADD antibody [FD19] (ab10519)
This product has been referenced in:
- Panner Aet al. mTOR-independent translational control of the extrinsic cell death pathway by RalA. Mol Cell Biol 26:7345-57 (2006). WB; Human.Read more (PubMed: 16894031) »
See 1 publication for this product
Publishing research using ab10519? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"