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Anti-FADD antibody (ab24533)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab24533 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

2 questions for ab24533

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Question 1

Friday 19-January-2007

I am trying to source an antibody that works against pig FADD. We have already tried the Stressgen antibody raised against the peptide from the human sequence KIDSIEDRYPPNLTER, and it did not work on pig cell lysates (contrary to the data sheet) but worked on human cell lysates. Upon closer examination, I found that the pig sequence is closer to the mouse peptide in this region and there are quite a number of differece between the pig and human sequences.

The abcam antibody ab24533 is raised against a peptide that includes this region, but your website only gives a range for the peptide of aa 100-150 and no precise sequence info. Does your peptide include the region ICDNVGKDWRRLARQLKVSD, which is immediately N terminal to the KIDSIEDRYPPNLTER sequence and a perfect match with the pig (Sus scrofa) sequence? I am basically wanting to know whether or not there is a chance that the abcam antibody will work on pig cells. Also, the dog and pig sequences are almost identical.

Thank you

ANSWER:

  

Thank you for your patience in this matter.

I have received a reply from the originator of ab24533 (FADD antibody). The immunogenic peptide sequence used in raising this product is: KIDSIEDRYPRNLTERVRESL.

Please do not hesitate to contact us again if you have further questions.

Question 2

Wednesday 25-January-2006

What residues does it correspond to on FADD? The product sheet states that it surrounds aa 128 of human FADD. I'm not sure exactly which residues it corresponds to.

ANSWER:

  

Thank you for your recent enquiry. I have been informed that the immunogen sequence is within the range of amino acids 100-150 of human FADD, I am unable to provide the specific immunogen sequence as this information is confidential.

I hope this information will help you, please do not hesitate to contact us again if you need further assistance,

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