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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-FADD antibody
See all FADD products (10) ...
Description
Rabbit polyclonal to FADD
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A region within synthetic peptide: AHLVGALRSC QMNLVADLVQ EVQQARDLQN RSGAMSPMSW NSDASTSEAS, corresponding to amino acids 159-208 of Human FADD
AHLVGALRSC QMNLVADLVQ EVQQARDLQN RSGAMSPMSW NSDASTSEAS
Positive control
Fetal thymus lysate, human kidney tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> FADD
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab48577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/62500ELISA: 1/62500
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificity
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similarities
Contains 1 death domain.
Contains 1 DED (death effector) domain.
Domain
Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
Target information above from: UniProt accessionQ13158
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Database links
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
Anti-FADD antibody images:
Western blot - FADD antibody (ab48577)
Anti-FADD antibody (ab48577) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + Fetal thymus lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 23 kDa
Observed band size : 23 kDa
Immunohistochemistry (Paraffin-embedded sections) - FADD antibody (ab48577)
ab48577 at 4.0ug/ml concentration staining human FADD in epithelial cells of renal tubule by Immunohistochemistry, Paraffin embedded tissue. Arrows indicate positively stained epithelial cells of renal tube.
References for Anti-FADD antibody (ab48577)
ab48577 has not yet been referenced specifically in any publications.
Publishing research using ab48577? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"