Recombinant Anti-FADS1 antibody [EPR6898] (ab126706)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6898] to FADS1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FADS1 antibody [EPR6898]
See all FADS1 primary antibodies -
Description
Rabbit monoclonal [EPR6898] to FADS1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human FADS1 aa 100-200. The exact sequence is proprietary.
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Positive control
- HepG2, Human fetal lung, fetal liver and fetal brain lysates; Human heart tissue; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6898 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively. -
Tissue specificity
Expressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina. -
Pathway
Lipid metabolism; polyunsaturated fatty acid biosynthesis. -
Sequence similarities
Belongs to the fatty acid desaturase family.
Contains 1 cytochrome b5 heme-binding domain. -
Developmental stage
Highly expressed in fetal liver and fetal brain. -
Domain
The histidine box domains may contain the active site and/or be involved in metal ion binding. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 3992 Human
- Entrez Gene: 76267 Mouse
- Entrez Gene: 84575 Rat
- Omim: 606148 Human
- SwissProt: O60427 Human
- SwissProt: Q920L1 Mouse
- SwissProt: Q920R3 Rat
- Unigene: 503546 Human
see all -
Alternative names
- D5D antibody
- Delta 5 desaturase antibody
- Delta 5 fatty acid desaturase antibody
see all
Images
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All lanes : Anti-FADS1 antibody [EPR6898] (ab126706) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal brain lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa -
Overlay histogram showing HepG2 cells stained with ab126706 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126706, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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ab126706, at 1/100 dilution, staining FADS1 in Paraffin-embedded Human heart tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab126706, at 1/100 dilution, staining FADS1 in A549 cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab126706 has been referenced in 17 publications.
- Xiao Y et al. Chromatin conformation of human oral epithelium can identify orofacial cleft missing functional variants. Int J Oral Sci 14:43 (2022). PubMed: 36008388
- Yu M et al. Berberine alleviates lipid metabolism disorders via inhibition of mitochondrial complex I in gut and liver. Int J Biol Sci 17:1693-1707 (2021). PubMed: 33994854
- Thomas T et al. Fatty acid desaturase activity in mature red blood cells and implications for blood storage quality. Transfusion 61:1867-1883 (2021). PubMed: 33904180
- Zhu L et al. Expressing the Human Cholesteryl Ester Transfer Protein Minigene Improves Diet-Induced Fatty Liver and Insulin Resistance in Female Mice. Front Physiol 12:799096 (2021). PubMed: 35082691
- Athinarayanan S et al. Fatty Acid Desaturase 1 Influences Hepatic Lipid Homeostasis by Modulating the PPARa-FGF21 Axis. Hepatol Commun 5:461-477 (2021). PubMed: 33681679