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Western blot - FADS1 antibody (ab59031)
(enlarge)
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Product Name
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FADS1 antibody
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See all FADS1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to FADS1
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Immunogen
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Synthetic peptide: C-QPSFEPTKNKELTDE, corresponding to internal sequence amino acids 97-111 of Human FADS1
BLAST 'C-QPSFEPTKNKELTDE' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Bb, Dog
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1:128,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Lung lysate.
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Cellular localization
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Endoplasmic reticulum membrane; Multi pass membrane protein
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
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Relevance
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FADS1 is a component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids from precursor essential polyunsaturated fatty acids, linoleic acid and alphanlinolenic acid. It catalyzes the desaturation of dihomo gamma linoleic acid and eicosatetraenoic acid to generate arachidonic acid and eicosapentaenoic acid respectively.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FADS1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FADS1 antibody images, references, products related to ab59031 and other tools.
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FADS1 antibody images:
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 Western blot - FADS1 antibody (ab59031)
FADS1 antibody (ab59031) at 0.3 µg/ml + Human Lung lysate (35µg protein in RIPA buffer)
Predicted band size : 52 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to FADS1
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FADS1 antibody - more information
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Customer reviews (feedback) regarding FADS1 antibody |
Customer FAQs regarding FADS1 antibody |
Protocols for FADS1 antibody |
Price and availability of products related to FADS1 antibody |
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FADS1 antibody - related products:
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FADS1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FADS1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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