Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Overview
Product name
Anti-FADS1 antibody
See all FADS1 products (4) ...
Description
Goat polyclonal to FADS1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog, Baboon
Immunogen
Synthetic peptide: C-QPSFEPTKNKELTDE, corresponding to internal sequence amino acids 97-111 of Human FADS1
C-QPSFEPTK NKELTDE
Positive control
Human Lung lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - FADS1 antibody (ab59031)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Peptide ELISA: antibody detection limit dilution 1:128,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively.
Tissue specificity
Expressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina.
Pathway
Lipid metabolism; polyunsaturated fatty acid biosynthesis.
Sequence similarities
Belongs to the fatty acid desaturase family.
Contains 1 cytochrome b5 heme-binding domain.
Developmental stage
Highly expressed in fetal liver and fetal brain.
Domain
The histidine box domains may contain the active site and/or be involved in metal ion binding.
Cellular localization
Endoplasmic reticulum membrane.
Target information above from: UniProt accessionO60427
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D5D antibody
- Delta 5 desaturase antibody
- Delta 5 fatty acid desaturase antibody
see all
Database links
- Entrez Gene: 100126707 Baboon
- Entrez Gene: 483793 Dog
- Entrez Gene: 3992 Human
- Entrez Gene: 76267 Mouse
- Entrez Gene: 84575 Rat
- Omim: 606148 Human
- SwissProt: A4UVI1 Baboon
- SwissProt: O60427 Human
see all
Anti-FADS1 antibody images:
Western blot - FADS1 antibody (ab59031)
Anti-FADS1 antibody (ab59031) at 0.3 µg/ml + Human Lung lysate (35µg protein in RIPA buffer)
Predicted band size : 52 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-FADS1 antibody (ab59031)
ab59031 has not yet been referenced specifically in any publications.
Publishing research using ab59031? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"