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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - FADS1 antibody (ab77312)
Anti-FADS1 antibody (ab77312) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 52 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant tagged fragment) was used for the test lane.
Western blot - FADS1 antibody (ab77312)
Anti-FADS1 antibody (ab77312) at 5 µg/ml + HeLa nuclear extract at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 52 kDa
Observed band size : 52 kDa
Sandwich ELISA - FADS1 antibody (ab77312)
Detection limit for recombinant tagged FADS1 is approximately 0.03ng/ml as a capture antibody
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