|
|
|
|
Western blot - FADS1 antibody (ab77312)
(enlarge)
|
|
Western blot - FADS1 antibody (ab77312)
(enlarge)
|
|
Sandwich ELISA - FADS1 antibody (ab77312)
(enlarge)
|
|
|
|
|
Product Name
|
FADS1 antibody
|
| |
See all FADS1 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal to FADS1
|
|
Immunogen
|
Recombinant fragment: MAPDPVAAET AAQGPTPRYF TWDEVAQRSG CEERWLVIDR KVYNISEFTR RHPGGSRVIS HYAGQDATDP FVAFHINKGL VKKYMNSLLI GELSPEQPS, corresponding to amino acids 1-99 of Human FADS1 (NP_037534) with a 26kDa tag.
BLAST 'MAPDPVAAET AAQGPTPRYF TWDEVAQRSG CEERWLVIDR KVYNISEFTR RHPGGSRVIS HYAGQDATDP FVAFHINKGL VKKYMNSLLI ' with  or 
|
|
Reacts with
|
Hu, RecFrag
|
|
Predicted to react
(species key)
|
Pig
(Due to sequence homology)
|
|
Tested applications
(see key)
|
ELISA, WB, sELISA
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution.
sELISA: Use at an assay dependent dilution. Detection limit is approximately 0.03ng/ml as a capture antibody when used against the immunogen.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa nuclear extract
|
|
Cellular localization
|
Endoplasmic reticulum membrane; Multi pass membrane protein
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
|
|
Relevance
|
FADS1 is a component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids from precursor essential polyunsaturated fatty acids, linoleic acid and alphanlinolenic acid. It catalyzes the desaturation of dihomo gamma linoleic acid and eicosatetraenoic acid to generate arachidonic acid and eicosapentaenoic acid respectively.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Isotype
|
IgG2a
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: None
Constituents: 1X PBS, pH 7.2
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FADS1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for FADS1 antibody images, references, products related to ab77312 and other tools.
|
| |
FADS1 antibody images:
|
 Western blot - FADS1 antibody (ab77312)
FADS1 antibody (ab77312) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 52 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant tagged fragment) was used for the test lane.
|
| |
Western blot - FADS1 antibody (ab77312)
FADS1 antibody (ab77312) at 5 µg/ml + HeLa nuclear extract at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 52 kDa
Observed band size : 52 kDa
|
| |
Sandwich ELISA - FADS1 antibody (ab77312)
Detection limit for recombinant tagged FADS1 is approximately 0.03ng/ml as a capture antibody
|
| |
| |
|
Search PubMed (MEDLINE) for references to FADS1
|
| |
FADS1 antibody - more information
|
Customer reviews (feedback) regarding FADS1 antibody |
Customer FAQs regarding FADS1 antibody |
Protocols for FADS1 antibody |
Price and availability of products related to FADS1 antibody |
| |
FADS1 antibody - related products:
|
|
|
| |
FADS1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about FADS1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
