You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-FADS1 antibody (ab77312)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-FADS1 antibody
See all FADS1 products (4) ...

Description

Mouse monoclonal to FADS1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human, Recombinant Fragment

Predicted to work with

Pig

Immunogen

Recombinant fragment: MAPDPVAAET AAQGPTPRYF TWDEVAQRSG CEERWLVIDR KVYNISEFTR RHPGGSRVIS HYAGQDATDP FVAFHINKGL VKKYMNSLLI GELSPEQPS, corresponding to amino acids 1-99 of Human FADS1 (NP_037534) with a 26kDa tag.

MAPDPVAAET AAQGPTPRYF TWDEVAQRSG CEERWLVIDR KVYNISEFTR RHPGGSRVIS HYAGQDATDP FVAFHINKGL VKKYMNSLLI GELSPEQPS

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa nuclear extract

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Isotype

IgG2a

Light chain type

kappa

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER

  • Western blot - FADS1 antibody (ab77312)Western blot - FADS1 antibody (ab77312) image (enlarge)

  • Western blot - FADS1 antibody (ab77312)Western blot - FADS1 antibody (ab77312) image (enlarge)

  • Sandwich ELISA - FADS1 antibody (ab77312)Sandwich ELISA - FADS1 antibody (ab77312) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide2 Images

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

  • ShowHide1 Image

    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution. (Detection limit is approximately 0.03ng/ml as a capture antibody when used against the immunogen.)

Target

Function

Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively.

Tissue specificity

Expressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina.

Pathway

Lipid metabolism; polyunsaturated fatty acid biosynthesis.

Sequence similarities

Belongs to the fatty acid desaturase family.
Contains 1 cytochrome b5 heme-binding domain.

Developmental stage

Highly expressed in fetal liver and fetal brain.

Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Cellular localization

Endoplasmic reticulum membrane.

Target information above from: UniProt accessionO60427 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • D5D antibody
  • Delta 5 desaturase antibody
  • Delta 5 fatty acid desaturase antibody
  • Delta(5) desaturase antibody
  • Delta(5) fatty acid desaturase antibody
  • Delta-5 desaturase antibody
  • Fads1 antibody
  • FADS1_HUMAN antibody
  • FADS6 antibody
  • FADSD5 antibody
  • Fatty acid desaturase 1 antibody
  • FLJ38956 antibody
  • Linoleoyl CoA desaturase (delta 6 desaturase) like 1 antibody
  • LLCDL1 antibody
  • TU12 antibody
see all

Database links

Anti-FADS1 antibody images:

  Western blot - FADS1 antibody (ab77312)

Western blot - FADS1 antibody (ab77312)

Anti-FADS1 antibody (ab77312) at 5 µg/ml + immunogen at 0.2 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size : 52 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)


The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant tagged fragment) was used for the test lane.

  Western blot - FADS1 antibody (ab77312)

Western blot - FADS1 antibody (ab77312)

Anti-FADS1 antibody (ab77312) at 5 µg/ml + HeLa nuclear extract at 50 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size : 52 kDa
Observed band size : 52 kDa

  Sandwich ELISA - FADS1 antibody (ab77312)

Sandwich ELISA - FADS1 antibody (ab77312)

Detection limit for recombinant tagged FADS1 is approximately 0.03ng/ml as a capture antibody

References for Anti-FADS1 antibody (ab77312)

ab77312 has not yet been referenced specifically in any publications.

Publishing research using ab77312? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"