Anti-FAIM2 antibody (ab58912)

We are really struggling to get this antibody against FAIM2 to work by immunoblotting onto nitrocellulose. Please could you look at the attached PPT image which explains our data. We have also expressed this protein by in vitro translation and not managed to detect any protein with the antibody. Please could you advise us as to how the data sheet image of the antibody was produced. If we cannot get this to work we would like to request a refund/alternative product to try.                                                                            

ANSWER:

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

It may well be that the antibody has been damaged during storage or shipment. I understand that transfection system was used and the FAIM2 was tagged with GFP. As you may aware, the tag itself may obscure native interactions and inhibit the binding of the antibody to the target due to 3-D sterical hindrance.

After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions:

- Construct: Does the construct contain full sequence or fragment of the target protein? Have you checked the GFP (tag) with a specific antibody in Western blot?

- Positive control: It may be worth running a good positive control along the samples to see if the experimental conditions are fine. Human, rat or mouse brain lysates or EL4 (Mouse lymphoma cell line) Whole Cell Lysate can be used for this purpose.

- Detection system: Does the detection system work fine? Have you used it successfully with another primary antibody?

I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Depa

Dear Abcam, We are really struggling to get this antibody against FAIM2 to work by immunoblotting onto nitrocellulose. Please could you look at the attached PPT image which explains our data.  We have also expressed this protein by in vitro translation and not managed to detect any protein with the antibody.   Please could you advise us as to how the data sheet image of the antibody was produced. If we cannot get this to work we would like to request a refund/alternative product to try. Many thanks in advance, Regards,  

ANSWER:

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. I appreciate that you have already sent the WB image with some information. In order to be able to process your complaint properly, I would need some further details. Would you be so kind to complete the following form (attached as a word document).

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.