FAK overexpression 293T lysate (whole cell) (ab94321)
Overview
-
Product name
FAK overexpression 293T lysate (whole cell)
See all FAK lysates -
General notes
ab94321 is a 293T cell transfected lysate in which Human FAK has been transiently over-expressed using a pCMV-FAK plasmid. The lysate is provided in 1X Sample Buffer.
-
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Research areas
-
Background
Domain: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL. The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. Function: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. PTM: Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent dilution.
|
Notes |
---|
WB
Use at an assay dependent dilution. |
Images
-
ab94321 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-FAK antibody (ab55632) at 1/500 dilution
Lane 1 :FAK overexpression 293T lysate (whole cell) (ab94321)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab94321 has not yet been referenced specifically in any publications.