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FAK peptide (Tagged) (ab42618)

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Overview

Product name

FAK peptide (Tagged)
See all FAK products (7) ...

Protein description

recombinant human FAK (amino acids 411 to 686) with N-terminal GST tag, MW = 62kD

Properties

Purification notes

>50% by SDS-PAGE

Biological activity

Specific Activity : 100 U/mg. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30deg.C. Assay buffer : 50mM HEPES pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3um Na-orthovanadate, 0.1M ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate and 1ug/ml recombinant FAK.

Form

Liquid

Storage instructions

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

Concentration

Concentration information loading...

Additional notes

Source : Baculovirus infected Sf9 cells

Research areas

Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> FAK family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> FAK / PYK
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins

  • SDS-PAGE - FAK protein (ab42618)SDS-PAGE - FAK protein (ab42618) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab42618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Inhibition Assay

     

Application notes

Inhib: Use at an assay dependent dilution.
This antibody is useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Tissue specificity

Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.

Domain

The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.

Post-translational
modifications

Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.

Cellular localization

Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.

Target information above from: UniProt accessionQ05397 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

FAK peptide (Tagged) images:

  SDS-PAGE - FAK protein (ab42618)

SDS-PAGE - FAK protein (ab42618)

10 ug protein loaded. Protein ladder bands above and below where protein
runs are 62kDa and 47kDa, respectively.

References for FAK peptide (Tagged) (ab42618)

ab42618 has not yet been referenced specifically in any publications.

Publishing research using ab42618? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"