Anti-FAK (phospho Y397) antibody (ab4803)

Western blot - FAK (phospho Y397) antibody (ab4803)

Peptide Competition: Extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4^oC, then were incubated with 0.35 µg/mL ab4803 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-tyrosine containing peptide (3), a phosphopeptide derived from the corresponding region of Pyk2 (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4803 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

Peptide Competition: Extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.35 µg/mL ab4803 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-tyrosine containing peptide (3), a phosphopeptide derived from the corresponding region of Pyk2 (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4803 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

- FAK (phospho Y397) antibody (ab4803)

Detection of ab4803 in focal adhesions of human melanoma cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK (phospho Y397) antibody (ab4803)

ab4803 at 1/100 staining (renin) transgenic rat aorta sections (5 micron) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 12 hours. A Cy3 ® conjugated goat polyclonal antibody was used as the secondary.

This image is courtesy of an anonymous Abreview