Anti-FAM73A antibody (ab121532)
Key features and details
- Rabbit polyclonal to FAM73A
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM73A antibody -
Description
Rabbit polyclonal to FAM73A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to internal sequence amino acids 279-405 of Human FAM73A.
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Positive control
- ICC/IF: A-431 IHC-P: Human prostate, kidney, placenta, testis tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the FAM73 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 374986 Human
- SwissProt: Q8NAN2 Human
- Unigene: 632419 Human
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Alternative names
- DKFZp686M07166 antibody
- FA73A_HUMAN antibody
- fam73a antibody
see all
Images
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Immunohistochemical analysis of human testis tissue labeling FAM73A with ab121532 at 1/20 dilution. Moderate granular cytoplasmic positivity in Leydig cells.
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Immunohistochemical analysis of human placenta tissue labeling FAM73A with ab121532 at 1/20 dilution. Strong granular cytoplasmic positivity in trophoblastic cells.
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Immunohistochemical analysis of human kidney tissue labeling FAM73A with ab121532 at 1/20 dilution. Moderate granular cytoplasmic positivity in cells in tubules.
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Immunohistochemical analysis of human prostate tissue labeling FAM73A with ab121532 at 1/20 dilution. Moderate to strong granular cytoplasmic positivity in glandular cells.
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Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121532 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121532 has not yet been referenced specifically in any publications.