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Western blot - FANCA antibody (ab5063)
(enlarge)
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Product Name
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FANCA antibody
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See all FANCA antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FANCA
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Immunogen
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Synthetic peptide: SRSYDHSENSDLVFG-C conjugated to KLH, corresponding to amino acids 995 - 1009 of Human FANCA.
BLAST 'SRSYDHSENSDLVFG-C' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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Detects a band at 130kDa in HeLa cell lysate that we believe corresponds to FANCA.
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 163 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear (major) and cytoplasmic (minor)
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Relevance
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FANCA is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It may be involved in interstrand DNA cross link repair and in the maintenance of normal chromosome stability.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FANCA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FANCA antibody images, references, products related to ab5063 and other tools.
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FANCA antibody images:
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 Western blot - FANCA antibody (ab5063)
FANCA antibody (ab5063) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 163 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa,75 kDa. We are unsure as to the identity of these extra bands.
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References for FANCA antibody (ab5063)
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This product has been used in:
Wilson JB et al. FANCG promotes formation of a newly identified protein complex containing BRCA2, FANCD2 and XRCC3. Oncogene 27:3641-52 (2008). PubMed: 18212739
If you publish research using ab5063 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FANCA
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FANCA antibody - more information
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Customer reviews (feedback) regarding FANCA antibody |
Customer FAQs regarding FANCA antibody |
Protocols for FANCA antibody |
Price and availability of products related to FANCA antibody |
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FANCA antibody - related products:
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FANCA antibody - other tools:
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Contact Abcam with a Technical Enquiry about FANCA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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