Anti-FANCA antibody (ab97578)

Overview

• Product nameAnti-FANCA antibodySee all FANCA primary antibodies ...
• Description
  Rabbit polyclonal to FANCA
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant protein fragment corresponding to a region within amino acids 1 and 190 of FANCA (O15360).

• Positive controlA431, HeLa, 293T or H1299 cell lines.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Fanconi's Anemia

Applications

Target

• FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
• Involvement in diseaseDefects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
• Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.

Target information above from: UniProt accession O15360 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2175 Human
  • Omim: 607139 Human
  • SwissProt: O15360 Human
  • Unigene: 290154 Human

• Alternative names
  FA 1 antibodyFA antibodyFA H antibody

  FA1 antibodyFAA antibodyFACA antibodyFAH antibodyFanca antibodyFANCA_HUMAN antibodyFANCH antibodyFanconi anemia complementation group A antibodyFanconi anemia complementation group H antibodyFanconi anemia group A protein antibodyFanconi anemia type 1 antibodyMGC75158 antibodyProtein FACA antibody

  see all

Anti-FANCA antibody images

• 

  Western blot - FANCA antibody (ab97578)
  All lanes : Anti-FANCA antibody (ab97578) at 1/1000 dilution
  
  Lane 1 : A431 whole cell lysate
  Lane 2 : HeLa whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 163 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - FANCA antibody (ab97578)
  Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97578 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FANCA antibody (ab97578)
  Immunohistochemistry analysis of paraffin-embedded OV90 xenograft, using ab97578 at 1/500 dilution.