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Western blot - FANCC antibody (ab5065)
(enlarge)
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Product Name
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FANCC antibody
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See all FANCC antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FANCC
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Immunogen
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Synthetic peptide: CLINKEPQNSGSKLNS, corresponding to amino acids 96 - 112 of Human FANCC.
BLAST 'CLINKEPQNSGSKLNS' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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Detects a band at 60kDa in Hela cell lysate corresponding to FANCC.
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear (major) and cytoplasmic (minor).
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Relevance
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FANCC is a DNA repair protein that may operate in postreplication repair or a cell cycle checkpoint function. It may be implicated in interstrand DNA cross link repair and in the maintenance of normal chromosome stability. Hsp70 requires the cooperation of FANCC to suppress PKR activity and support survival of haematopoietic cells and FANCC does not require the multimeric Fanconi anemia complex to exert this function.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.450 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FANCC antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FANCC antibody images, references, products related to ab5065 and other tools.
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FANCC antibody images:
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 Western blot - FANCC antibody (ab5065)
FANCC antibody (ab5065) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size : 63 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.
Primary antibody incubated with PBS supplemented with 1% normal goat serum and 0.1% milk.
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Search PubMed (MEDLINE) for references to FANCC
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FANCC antibody - more information
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Customer reviews (feedback) regarding FANCC antibody |
Customer FAQs regarding FANCC antibody |
Protocols for FANCC antibody |
Price and availability of products related to FANCC antibody |
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FANCC antibody - related products:
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FANCC antibody - other tools:
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Contact Abcam with a Technical Enquiry about FANCC antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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