Anti-FANCC antibody (ab54631)

Western blot - FANCC antibody (ab54631)

Anti-FANCC antibody (ab54631) at 5 µg/ml + 0.2 µg/lane of recombinant protein with a tag

Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size : 63 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)


Note: The difference between predicted and actual band sizes may be due to the fact that ab54631 detects a partial recombinant protein, and not the full length protein..

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Western blot - FANCC antibody (ab54631)

All lanes : Anti-FANCC antibody (ab54631) at 2 µg/ml

Lane 1 : whole cell lysate prepared from human fibroblasts
Lane 2 : whole cell lysate prepared from human fibroblasts
Lane 3 : whole cell lysate prepared from human fibroblasts
Lane 4 : whole cell lysate prepared from human fibroblasts

Lysates/proteins at 40 µg per lane.

Secondary
HRP conjugated sheep anti-mouse IgG at 1/2000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 63 kDa
Observed band size : 63 kDa


Exposure time : 3 minutes

Gel was run under denaturing conditions using 12% Bis-Tris, MES buffer.
Primary antibody incubated for 16 hours at +4°C.

Image courtesy of Daniel Meier by Abreview

Immunocytochemistry/ Immunofluorescence-FANCC antibody(ab54631)

ICC/IF image of ab54631 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54631, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.