Overview

  • Product nameAnti-FANCC antibody
    See all FANCC primary antibodies
  • Description
    Mouse monoclonal to FANCC
  • Tested applicationsWB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: MAQDSVDLSC DYQFWMQKLS VWDQASTLET QQDTCLHVAQ FQEFLRKMYE ALKEMDSNTV IERFPTIGQL LAKACWNPFI LAYDESQKIL IWCLCCLINK , corresponding to amino acids 1-101 of Human FANCC

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab54631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • Developmental stageExpression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
  • Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA80I15.1 antibody
    • FA 3 antibody
    • FA3 antibody
    • FAC antibody
    • FACC antibody
    • FANCC antibody
    • FANCC_HUMAN antibody
    • Fanconi anemia complementation group C antibody
    • Fanconi anemia complementation group C protein antibody
    • Fanconi anemia group C protein antibody
    • Fanconi pancytopenia type 3 antibody
    • FLJ14675 antibody
    • Protein FACC antibody
    see all

Anti-FANCC antibody images

  • Anti-FANCC antibody (ab54631) at 5 µg/ml + 0.2 µg/lane of recombinant protein with a tag

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FANCC antibody (ab54631) at 2 µg/ml

    Lane 1 : whole cell lysate prepared from human fibroblasts
    Lane 2 : whole cell lysate prepared from human fibroblasts
    Lane 3 : whole cell lysate prepared from human fibroblasts
    Lane 4 : whole cell lysate prepared from human fibroblasts

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP conjugated sheep anti-mouse IgG at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa


    Exposure time : 3 minutes

    Image courtesy of Daniel Meier by Abreview

    See Abreview

  • ICC/IF image of ab54631 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54631, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab54631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-FANCC antibody (ab54631)

ab54631 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Fibroblasts)
Loading amount 40 µg
Specification Fibroblasts
Gel Running Conditions Reduced Denaturing (12% Bis-Tris, MES Buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Herr Dr. Daniel Meier

Verified customer

Submitted Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"