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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
Overview
Product name
Anti-FANCC antibody
See all FANCC products (5) ...
Description
Mouse monoclonal to FANCC
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human, Recombinant Fragment
Immunogen
Recombinant fragment: MAQDSVDLSC DYQFWMQKLS VWDQASTLET QQDTCLHVAQ FQEFLRKMYE ALKEMDSNTV IERFPTIGQL LAKACWNPFI LAYDESQKIL IWCLCCLINK , corresponding to amino acids 1-101 of Human FANCC
MAQDSVDLSC DYQFWMQKLS VWDQASTLET QQDTCLHVAQ FQEFLRKMYE ALKEMDSNTV IERFPTIGQL LAKACWNPFI LAYDESQKIL IWCLCCLINK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 63 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Relevance
FANCC is a DNA repair protein that may operate in postreplication repair or a cell cycle checkpoint function. It may be implicated in interstrand DNA cross link repair and in the maintenance of normal chromosome stability. Hsp70 requires the cooperation of FANCC to suppress PKR activity and support survival of haematopoietic cells and FANCC does not require the multimeric Fanconi anemia complex to exert this function.
Cellular localization
Nuclear (major) and cytoplasmic (minor).
Alternative names
- Fanconi anemia group C protein antibody
- bA80I15.1 antibody
- FA 3 antibody
see all
Database links
- Entrez Gene: 2176 Human
- Omim: 227645 Human
- SwissProt: Q00597 Human
Anti-FANCC antibody images:
Western blot - FANCC antibody (ab54631)
Anti-FANCC antibody (ab54631) at 5 µg/ml + 0.2 µg/lane of recombinant protein with a tag
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size : 63 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Note: The difference between predicted and actual band sizes may be due to the fact that ab54631 detects a partial recombinant protein, and not the full length protein..
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Western blot - FANCC antibody (ab54631)
All lanes : Anti-FANCC antibody (ab54631) at 2 µg/ml
Lane 1 : whole cell lysate prepared from human fibroblasts
Lane 2 : whole cell lysate prepared from human fibroblasts
Lane 3 : whole cell lysate prepared from human fibroblasts
Lane 4 : whole cell lysate prepared from human fibroblasts
Lysates/proteins at 40 µg per lane.
Secondary
HRP conjugated sheep anti-mouse IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 63 kDa
Observed band size : 63 kDa
Exposure time : 3 minutes
Gel was run under denaturing conditions using 12% Bis-Tris, MES buffer.Primary antibody incubated for 16 hours at +4°C.
Image courtesy of Daniel Meier by Abreview
Immunocytochemistry/ Immunofluorescence-FANCC antibody(ab54631)
ICC/IF image of ab54631 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54631, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-FANCC antibody (ab54631)
ab54631 has not yet been referenced specifically in any publications.
Publishing research using ab54631? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"