Anti-FANCC antibody (ab54631)

Overview

• Product nameAnti-FANCC antibodySee all FANCC primary antibodies ...
• Description
  Mouse monoclonal to FANCC
• Tested applicationsWB, ICC/IF, Flow Cyt more details
• Species reactivity
  Reacts with: Human, Recombinant Fragment
  
• Immunogen

  Recombinant fragment: MAQDSVDLSC DYQFWMQKLS VWDQASTLET QQDTCLHVAQ FQEFLRKMYE ALKEMDSNTV IERFPTIGQL LAKACWNPFI LAYDESQKIL IWCLCCLINK , corresponding to amino acids 1-101 of Human FANCC

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Fanconi's Anemia

Applications

Target

• FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
• Tissue specificityUbiquitous.
• Involvement in diseaseDefects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
• Developmental stageExpression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
• Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.

Target information above from: UniProt accession Q00597 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2176 Human
  • Entrez Gene: 2176 Human
  • Entrez Gene: 2176 Human
  • Omim: 613899 Human
  • SwissProt: Q00597 Human
  • SwissProt: Q00597 Human
  • SwissProt: Q00597 Human
  • Unigene: 494529 Human

• Alternative names
  bA80I15.1 antibodyFA 3 antibodyFA3 antibody

  FAC antibodyFACC antibodyFANCC antibodyFANCC_HUMAN antibodyFanconi anemia complementation group C antibodyFanconi anemia complementation group C protein antibodyFanconi anemia group C protein antibodyFanconi pancytopenia type 3 antibodyFLJ14675 antibodyProtein FACC antibody

  see all

Anti-FANCC antibody images

• 

  Western blot - FANCC antibody (ab54631)
  Anti-FANCC antibody (ab54631) at 5 µg/ml + 0.2 µg/lane of recombinant protein with a tag
  
  Secondary
  Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
  
  Predicted band size : 63 kDa
  Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - FANCC antibody (ab54631)
  All lanes : Anti-FANCC antibody (ab54631) at 2 µg/ml
  
  Lane 1 : whole cell lysate prepared from human fibroblasts
  Lane 2 : whole cell lysate prepared from human fibroblasts
  Lane 3 : whole cell lysate prepared from human fibroblasts
  Lane 4 : whole cell lysate prepared from human fibroblasts
  
  Lysates/proteins at 40 µg per lane.
  
  Secondary
  HRP conjugated sheep anti-mouse IgG at 1/2000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa
  
  
  Exposure time : 3 minutes

  Image courtesy of Daniel Meier by Abreview

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence-FANCC antibody(ab54631)
  ICC/IF image of ab54631 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54631, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Flow Cytometry-Anti-FANCC antibody(ab54631)
  Overlay histogram showing HeLa cells stained with ab54631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54631, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.