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Western blot - FANCD2 antibody [103 ] (ab12450)
![Western blot - FANCD2 antibody [103 ] (ab12450)](/ps/datasheet/images/ab12450_2.jpg)
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Product Name
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FANCD2 antibody [103 ]
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See all FANCD2 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [103 ] to FANCD2
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Immunogen
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Human FANCD2 fusion protein
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution PMID 17643815.
WB: 1/500 - 1/1000. Detects a band of approximately 155, 170 kDa (predicted molecular weight: 176.5 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Relevance
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Fanconi Anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANC D2, targeting nuclear foci containing the BRCA 1 protein.
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FANCD2 antibody [103 ] (ab12450) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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103
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Myeloma
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NS1
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Purification notes
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Affinity purified from mouse ascitic fluid.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FANCD2 antibody [103 ] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FANCD2 antibody [103 ] images, references, products related to ab12450 and other tools.
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FANCD2 antibody [103 ] images:
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 Western blot - FANCD2 antibody [103 ] (ab12450)
Predicted band size : 176.5 kDa
ab12450 staining human FANCD2 in HeLa whole cell lysate by Western blot (ECL).
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References for FANCD2 antibody [103 ] (ab12450)
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This product has been used in:
Lyakhovich A & Surralles J FANCD2 depletion sensitizes cancer cells repopulation ability in vitro. Cancer Lett 256:186-95 (2007). ICC/IF; Human. PubMed: 17643815
If you publish research using ab12450 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FANCD2
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FANCD2 antibody [103 ] - more information
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Customer reviews (feedback) regarding FANCD2 antibody [103 ] |
Customer FAQs regarding FANCD2 antibody [103 ] |
Protocols for FANCD2 antibody [103 ] |
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FANCD2 antibody [103 ] - related products:
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FANCD2 antibody [103 ] - other tools:
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Contact Abcam with a Technical Enquiry about FANCD2 antibody [103 ] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - FANCD2 antibody [103 ] (ab12450)](/index.html?datasheet=12450#ab12450_2.jpg)
