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Immunoprecipitation - FANCD2 antibody [FI-17] (ab5360)
![Immunoprecipitation - FANCD2 antibody [FI-17] (ab5360)](/ps/datasheet/images/ab5360_1.jpg)
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Product Name
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FANCD2 antibody [FI-17] - ChIP Grade
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See all FANCD2 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [FI-17] to FANCD2 - ChIP Grade
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Immunogen
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FANCD2 fusion protein (Human).
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Hm
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Specificity
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Specific for human FANCD2.
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Tested applications
(see key)
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ChIP, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 19748364).
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/2000. Detects a band of approximately 155 and 162 kDa (predicted molecular weight: 166 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Relevance
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Fanconi Anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANC D2, targeting nuclear foci containing the BRCA 1 protein.
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FANCD2 antibody [FI-17] - ChIP Grade (ab5360) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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FI-17
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Raw Ascites Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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34.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FANCD2 antibody [FI-17] - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FANCD2 antibody [FI-17] - ChIP Grade images, references, products related to ab5360 and other tools.
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FANCD2 antibody [FI-17] - ChIP Grade images:
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 Immunoprecipitation - FANCD2 antibody [FI-17] (ab5360)
Samples were immunoprecipitated with ab5360 (4ug antibody/1mg cell lysate)
FANCD2-S and -L were clearly seen in the figure. Left lane is non-irradiated samples while right lane is sample irradiated with 5 Gray (Gy) ionizing irradiation. FANCD2-L is seen in non-irradiated sample due to S-phase progression. This image is courtesy of an anonymous Abreview
See Abreview
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References for FANCD2 antibody [FI-17] - ChIP Grade (ab5360)
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This product has been used in: (two most recent references)
Shen X et al. Recruitment of fanconi anemia and breast cancer proteins to DNA damage sites is differentially governed by replication. Mol Cell 35:716-23 (2009). ChIP; Human. PubMed: 19748364
Garcia-Higuera I et al. Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway. Mol Cell 7:249-62 (2001). PubMed: 11239454
If you publish research using ab5360 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FANCD2
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FANCD2 antibody [FI-17] - ChIP Grade - more information
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Customer reviews (feedback) regarding FANCD2 antibody [FI-17] - ChIP Grade |
Customer FAQs regarding FANCD2 antibody [FI-17] - ChIP Grade |
Protocols for FANCD2 antibody [FI-17] - ChIP Grade |
Price and availability of products related to FANCD2 antibody [FI-17] - ChIP Grade |
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FANCD2 antibody [FI-17] - ChIP Grade - related products:
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FANCD2 antibody [FI-17] - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about FANCD2 antibody [FI-17] - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunoprecipitation - FANCD2 antibody [FI-17] (ab5360)](/index.html?datasheet=5360#ab5360_1.jpg)
