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ab31576
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab31577? Please let us know so that we can cite the reference in this datasheet
ab31577 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - FANCD2 antibody (ab31577)
All lanes : Anti-FANCD2 antibody (ab31577) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 :
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 166 kDa
Observed band size : 166 kDa
Additional bands at : 37 kDa (possible cleavage fragment,cross reactivity).
Immunocytochemistry/ Immunofluorescence - FANCD2 antibody (ab31577)
ab31577 (1/200) staining FANCD2 in HeLa cells (green). Cells were fixed in methanol, permeabilised with 0.5% Triton-X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
Western blot - FANCD2 antibody (ab31577)
Anti-FANCD2 antibody (ab31577) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 166 kDa
Observed band size : 166 kDa
Additional bands at : 110 kDa,41 kDa,71 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
FANCD2 antibody for ICC/IF in Human (31577)
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