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Western blot - FANCD2 antibody (ab31577)
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Immunocytochemistry/ Immunofluorescence - FANCD2 antibody (ab31577)
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Product Name
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FANCD2 antibody
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See all FANCD2 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FANCD2
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Immunogen
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Synthetic peptide derived from within residues 1200 - 1300 of Human FANCD2.
(Peptide available as ab31576.)
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Reacts with
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Hu
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: 1/200.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Relevance
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Fanconi Anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANC D2, targeting nuclear foci containing the BRCA 1 protein.
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FANCD2 antibody (ab31577) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.016% Sodium Azide
Constituents: 0.8% BSA, PBS, 80mM Boric Acid, 60mM Sodium chloride, 4mM EDTA, 20mM Sodium borate, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.80 - 1.20 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FANCD2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FANCD2 antibody images, references, products related to ab31577 and other tools.
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FANCD2 antibody images:
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 Immunocytochemistry/ Immunofluorescence - FANCD2 antibody (ab31577)
ab31577 (1/200) staining FANCD2 in HeLa cells (green). Cells were fixed in methanol, permeabilised with 0.5% Triton-X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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Search PubMed (MEDLINE) for references to FANCD2
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FANCD2 antibody - more information
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Customer reviews (feedback) regarding FANCD2 antibody |
Customer FAQs regarding FANCD2 antibody |
Protocols for FANCD2 antibody |
Price and availability of products related to FANCD2 antibody |
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FANCD2 antibody - related products:
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FANCD2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FANCD2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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