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Western blot - FANCD2 antibody (ab31577)
Western blot - FANCD2 antibody (ab31577)
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Immunocytochemistry/ Immunofluorescence - FANCD2 antibody (ab31577)
Immunocytochemistry/ Immunofluorescence - FANCD2 antibody (ab31577)
(enlarge)


Product Name 

FANCD2 antibody

 

See all FANCD2 antibodies (11)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FANCD2

Immunogen 

Synthetic peptide derived from within residues 1200 - 1300 of Human FANCD2.

(Peptide available as ab31576.)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
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• ICC/IF

1 Abreview

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ICC/IF
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/200.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia

Relevance 

Fanconi Anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANC D2, targeting nuclear foci containing the BRCA 1 protein.

 

FANCD2 antibody (ab31577)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2177    (Human)

Omim

    

227646    (Human)

SwissProt

    

Q6PJN7    (Human)

Unigene

    

208388    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.016% Sodium Azide
Constituents: 0.8% BSA, PBS, 80mM Boric Acid, 60mM Sodium chloride, 4mM EDTA, 20mM Sodium borate, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.80 - 1.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FANCD2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for FANCD2 antibody images, references, products related to ab31577 and other tools.

 

FANCD2 antibody images:

Product image  Western blot - FANCD2 antibody (ab31577)

Western blot - FANCD2 antibody (ab31577)

All lanes : FANCD2 antibody (ab31577) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 166 kDa
Observed band size : 166 kDa
Additional bands at : 37 kDa (possible cleavage fragment,cross reactivity).

 

Product image  Immunocytochemistry/ Immunofluorescence - FANCD2 antibody (ab31577)

Immunocytochemistry/ Immunofluorescence - FANCD2 antibody (ab31577)

ab31577 (1/200) staining FANCD2 in HeLa cells (green). Cells were fixed in methanol, permeabilised with 0.5% Triton-X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

This image is courtesy of an Abreview submitted by Dr Kirk McManus

See Abreview

 
 

Search PubMed (MEDLINE) for references to FANCD2

 

FANCD2 antibody - more information

Customer reviews (feedback) regarding FANCD2 antibody

Customer FAQs regarding FANCD2 antibody

Protocols for FANCD2 antibody

Price and availability of products related to FANCD2 antibody

 

FANCD2 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for FANCD2 antibody

ab31576

  

FANCD2 peptide

 

Compatible Secondaries for FANCD2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other FANCD2 Antibodies

See all FANCD2 antibodies (11)...

 

FANCD2 antibody - other tools:

Contact Abcam with a Technical Enquiry about FANCD2 antibody

 

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