Anti-FANCG antibody (ab54645)

Overview

  • Product nameAnti-FANCG antibodySee all FANCG primary antibodies ...
  • Description
    Mouse monoclonal to FANCG
  • Tested applicationsWB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-623 of Human FANCG

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab54645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
Flow Cyt Use 0.5-1µg for 106 cells.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
  • Tissue specificityHighly expressed in testis and thymus. Found in lymphoblasts.
  • Involvement in diseaseDefects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • Sequence similaritiesContains 4 TPR repeats.
  • Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein XRCC9 antibody
    • DNA-repair protein XRCC9 antibody
    • FAG antibody
    • FANCG antibody
    • FANCG_HUMAN antibody
    • Fanconi anaemia complementation group G antibody
    • Fanconi anemia group G protein antibody
    • Protein FACG antibody
    • X ray repair, complementing defective, in Chinese hamster cells 9 antibody
    • X-ray repair, complementing defective, in Chinese hamster, 9 antibody
    • XRCC9 antibody
    see all

Anti-FANCG antibody images



  • Predicted band size : 69 kDa
    FANCG antibody (ab54645) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54645 at 10 µg/ml staining FANCG in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab54645 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54645, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-FANCG antibody (ab54645)

ab54645 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 2% TritonX100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: RT°C
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Submitted Mar 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"