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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
Overview
Product name
Anti-FANCG antibody
See all FANCG products (6) ...
Description
Goat polyclonal to FANCG
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Macaque Monkey
Immunogen
Synthetic peptide: LEEFRTSLPKSCDL, corresponding to C terminal amino acids 609-622 of Human FANCG
LEEFRTSLPK SCDL
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Western blot - FANCG antibody (ab77193)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
Tissue specificity
Highly expressed in testis and thymus. Found in lymphoblasts.
Involvement in disease
Defects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
Sequence similarities
Contains 4 TPR repeats.
Cellular localization
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
Target information above from: UniProt accessionO15287
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein XRCC9 antibody
- FAG antibody
- FANCG antibody
see all
Database links
- Entrez Gene: 2189 Human
- Omim: 602956 Human
- SwissProt: O15287 Human
- Unigene: 591084 Human
Anti-FANCG antibody images:
Western blot - FANCG antibody (ab77193)
Anti-FANCG antibody (ab77193) at 0.5 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 69 kDa
Observed band size : 69 kDa
Exposure time : 1 hour
References for Anti-FANCG antibody (ab77193)
ab77193 has not yet been referenced specifically in any publications.
Publishing research using ab77193? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"