Application notesThis antibody gave a positive result in ELISA against the immunizing peptide (ab33127). It gave a negative result in ELISA against the non-modified equivalent peptide (ab42660). This indicates that it is specific for the modified peptide.
See figure below.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
Tissue specificityHighly expressed in testis and thymus. Found in lymphoblasts.
Involvement in diseaseDefects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
Sequence similaritiesContains 4 TPR repeats.
Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
This antibody gave a positive result in ELISA against the immunizing peptide (ab33127, blue). It gave a negative result in ELISA against the non-modified equivalent peptide (ab42660, purple). This indicates that it is specific for the modified peptide.
CC/IF image of ab31893 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab31893, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.However, this Fast-Track antibody is not yet fully characterised.This image represents inconclusive preliminary data.
References for Anti-FANCG (phospho S383) antibody (ab31893)
has not yet been referenced specifically in any publications.
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