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Product Name
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FANCL antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FANCL
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human FANCL. (Peptide available as ab42702.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Cow, Orngt
(Due to sequence homology)
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF/ICC: see image below
This antibody gave a positive result in ELISA against the immunizing peptide
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Relevance
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FANCL is a ubiquitin ligase protein that mediates ubiquitination of FANCD2, a key step in the DNA damage pathway.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.48 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FANCL antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FANCL antibody images, references, products related to ab42639 and other tools.
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FANCL antibody images:
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This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'
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 Immunocytochemistry/ Immunofluorescence - FANCL antibody (ab42639)
ICC/IF image of ab42639 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42639, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa cells fixed in 4% PFA and HepG2, MCF7 and Hek293 cells fixed in both 4% PFA and 100% methanol, all at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
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Search PubMed (MEDLINE) for references to FANCL
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FANCL antibody - more information
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Customer reviews (feedback) regarding FANCL antibody |
Customer FAQs regarding FANCL antibody |
Protocols for FANCL antibody |
Price and availability of products related to FANCL antibody |
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FANCL antibody - related products:
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FANCL antibody - other tools:
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Contact Abcam with a Technical Enquiry about FANCL antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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