Overview

  • Product nameAnti-FANCM antibody
    See all FANCM primary antibodies
  • Description
    Rabbit polyclonal to FANCM
  • Tested applicationsIP, WBmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human FANCM.

    (Peptide available as ab27788.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates; HeLa, MDA-MB-361 and WI38.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab26272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1 µg/mg of lysate. (see Abreview)
WB Use a concentration of 1 mg/ml. Detects a band of approximately 240 kDa (predicted molecular weight: 232 kDa).Can be blocked with Human FANCM peptide (ab27788).

Target

  • RelevanceFANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ATP-dependent RNA helicase FANCM antibody
    • FAAP250 antibody
    • Fanconi anemia group M protein antibody
    • fanconi anemia-associated polypeptide of 250 kDa antibody
    • KIAA1596 antibody
    • Protein Hef ortholog antibody
    see all

Anti-FANCM antibody images

  • All lanes : Anti-FANCM antibody (ab26272) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 232 kDa
    Observed band size : 238 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-FANCM antibody (ab26272)

ab26272 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Breast, Cal51)
Loading amount 500 cells
Specification Breast, Cal51
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Username

Mr. Richard Allum

Verified customer

Submitted Oct 06 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"