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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
Overview
Product name
Anti-FANCM antibody
See all FANCM products (5) ...
Description
Rabbit polyclonal to FANCM
Tested applications
IP, WBmore details
Cross reactivity
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human FANCM.
(Peptide available as ab27788.)
Positive control
This antibody gave a positive signal in the following whole cell lysates; HeLa, MDA-MB-361 and WI38.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Western blot - FANCM antibody (ab26272)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 1 µg/mg of lysate. ((see Abreview))
WB: Use a concentration of 1 mg/mlDetects a band of approximately 240 kDa (predicted molecular weight: 232 kDa).
Target
Relevance
FANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA.
Cellular localization
Nuclear
Alternative names
- FAAP250 antibody
- Fanconi anemia group M protein antibody
- Protein Hef ortholog antibody
Database links
- Entrez Gene: 57697 Human
- Omim: 609644 Human
- SwissProt: Q8IYD8 Human
Anti-FANCM antibody images:
Western blot - FANCM antibody (ab26272)
All lanes : Anti-FANCM antibody (ab26272) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 232 kDa
Observed band size : 238 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
References for Anti-FANCM antibody (ab26272)
ab26272 has not yet been referenced specifically in any publications.
Publishing research using ab26272? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"