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Anti-FANCM antibody (ab35620)

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Overview

Product name

Anti-FANCM antibody
See all FANCM products (5) ...

Description

Rabbit polyclonal to FANCM

Tested applications

WB, IPmore details

Cross reactivity

Predicted to work with

Human

Immunogen

Synthetic peptide derived from within residues 2000 to the C-terminus of Human FANCM.

Read Abcam's proprietary immunogen policy

(Peptide available as ab27787.)

Positive control

This antibody gave a positive signal in Hek293 whole cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia

  • Western blot - FANCM antibody (ab35620)Western blot - FANCM antibody (ab35620) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab35620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlDetects a band of approximately 248 kDa (predicted molecular weight: 232 kDa).

  • ShowHide

    IP

     IP: Use at 1 µg/mg of ly...Read more →

    IP: Use at 1 µg/mg of lysate. ((see Abreview), see also PMID 20064461. This antibody gave a positive result in ELISA against the immunizing peptide (ab27787).)

Target

Relevance

FANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA.

Cellular localization

Nuclear

Alternative names

  • FAAP250 antibody
  • Fanconi anemia group M protein antibody
  • Protein Hef ortholog antibody

Database links

Anti-FANCM antibody images:

  Western blot - FANCM antibody (ab35620)

Western blot - FANCM antibody (ab35620)

Anti-FANCM antibody (ab35620) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 232 kDa
Observed band size : 248 kDa (why is the actual band size different from the predicted?)
Additional bands at : 43 kDa,98 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 12 minutes

References for Anti-FANCM antibody (ab35620)

This product has been referenced in:

  • Deans AJ & West SC FANCM connects the genome instability disorders Bloom's Syndrome and Fanconi Anemia. Mol Cell 36:943-53 (2009). IP; Human.Read more (PubMed: 20064461) »

See 1 publication for this product

Publishing research using ab35620? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"