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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-FAU antibody
See all FAU products (5) ...
Description
Mouse polyclonal to FAU
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fusion protein containing the full length FAU precursor protein (Human)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Western blot - FAU antibody (ab43581)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab43581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 14 kDa. This is the predicted molecular weight of the unprocessed FAU precursor protein consisting of the ubiquitin-like protein Fubi at the N terminus and ribosomal protein S30 at the C terminus.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Belongs to the ubiquitin family.
Target information above from: UniProt accessionP35544
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 40S ribosomal protein S30 antibody
- asr1 antibody
- FAU antibody
see all
Database links
- Entrez Gene: 2197 Human
- Omim: 134690 Human
- SwissProt: P35544 Human
- SwissProt: P62861 Human
- Unigene: 387208 Human
Anti-FAU antibody images:
Western blot - FAU antibody (ab43581)
All lanes : Anti-FAU antibody (ab43581) at 1/1000 dilution
Lane 1 : Total protein extract from E.coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen
Lane 2 : Total protein extract from E.coli with ~50ng to 500ng of the antigen fusion protein
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 14 kDa
The predicted molecular weight is the molecular weight of the unprocessed FAU precursor protein consisting of the ubiquitin-like protein Fubi at the N terminus and ribosomal protein S30 at the C terminus.
The size of the protein band was not determined. The molecular weight of the band on the western blot does not correspond to the predicted molecular weight of the natural protein because of the additional mass of the fusion.
References for Anti-FAU antibody (ab43581)
ab43581 has not yet been referenced specifically in any publications.
Publishing research using ab43581? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"