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All lanes : Anti-FAU antibody (ab63063) at 1/100 dilution
Lane 1 : 293 cell lysate, nontransfected
Lane 2 : 293 cell lysate, transiently transfected with the FAU gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 14 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Ab63063 (1/50-1/100) staining human FAU in human colon carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Ab63063 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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