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Western blot - FAU antibody (ab63063)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAU antibody (ab63063)
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Product Name
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FAU antibody
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See all FAU antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FAU
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Immunogen
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KLH conjugated synthetic peptide selected from the N-terminal region of human FAU.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293 cell lysate or human colon carcinoma tissue.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Relevance
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FAU is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FAU antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FAU antibody images, references, products related to ab63063 and other tools.
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FAU antibody images:
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 Western blot - FAU antibody (ab63063)
All lanes : FAU antibody (ab63063) at 1/100 dilution
Lane 1 : 293 cell lysate, nontransfected
Lane 2 : 293 cell lysate, transiently transfected with the FAU gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 14 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAU antibody (ab63063)
Ab63063 (1/50-1/100) staining human FAU in human colon carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Ab63063 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Search PubMed (MEDLINE) for references to FAU
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FAU antibody - more information
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Customer reviews (feedback) regarding FAU antibody |
Customer FAQs regarding FAU antibody |
Protocols for FAU antibody |
Price and availability of products related to FAU antibody |
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FAU antibody - related products:
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FAU antibody - other tools:
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Contact Abcam with a Technical Enquiry about FAU antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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