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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-FAU antibody
See all FAU products (5) ...
Description
Rabbit polyclonal to FAU
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH conjugated synthetic peptide selected from the C-terminal region of human FAU.
Positive control
Human colon carcinoma tissue, Hela and T47D cell line lysates and 293 cell line lysates transiently transfected with the FAU gene.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab63065 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Sequence similarities
Belongs to the ubiquitin family.
Target information above from: UniProt accessionP35544
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 40S ribosomal protein S30 antibody
- asr1 antibody
- FAU antibody
see all
Database links
- Entrez Gene: 2197 Human
- Omim: 134690 Human
- SwissProt: P35544 Human
- SwissProt: P62861 Human
- Unigene: 387208 Human
Anti-FAU antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAU antibody (ab63065)
ab63065 at 1/50 - 1/100 dilution, staining FAU in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot - FAU antibody (ab63065)
Anti-FAU antibody (ab63065) at 1/60 dilution + HeLa cell lysate at 2µg per lane
Predicted band size : 14 kDa
Observed band size : 14 kDa
Western blot - FAU antibody (ab63065)
Anti-FAU antibody (ab63065) at 1/60 dilution + T47D cell lysate at 2µg per lane
Predicted band size : 14 kDa
Observed band size : 14 kDa
Western blot - FAU antibody (ab63065)
All lanes : Anti-FAU antibody (ab63065) at 1/60 dilution
Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate transiently transfected with the FAU gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 14 kDa
Observed band size : 14 kDa
References for Anti-FAU antibody (ab63065)
ab63065 has not yet been referenced specifically in any publications.
Publishing research using ab63065? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"