Anti-FBXL2 antibody (ab17018)
Key features and details
- Goat polyclonal to FBXL2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FBXL2 antibody
See all FBXL2 primary antibodies -
Description
Goat polyclonal to FBXL2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide: VFSNNDEGLINKK, corresponding to N terminal amino acids 2-14 of Human FBXL2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas and placenta. -
Sequence similarities
Contains 1 F-box domain.
Contains 13 LRR (leucine-rich) repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 616212 Cow
- Entrez Gene: 25827 Human
- Entrez Gene: 363156 Rat
- Omim: 605652 Human
- SwissProt: A6H779 Cow
- SwissProt: Q9UKC9 Human
- Unigene: 475872 Human
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Alternative names
- DKFZP564P0622 antibody
- F box and leucine rich repeat protein 2 antibody
- F box protein containing leucine rich repeats antibody
see all
Images
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All lanes : Anti-FBXL2 antibody (ab17018) at 0.3 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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ab17018 at 0.5
µ g/ml detecting FBXL2 from Human kidney lysate (35µ g total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab17018 has been referenced in 2 publications.
- Liao C et al. Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer. Cancer Discov 10:1706-1721 (2020). PubMed: 32690540
- Yu Q et al. miRNA-346 promotes proliferation, migration and invasion in liver cancer. Oncol Lett 14:3255-3260 (2017). PubMed: 28927074